Thermal stress induces heat shock protein 70 and apoptosis during embryo development in a Neotropical freshwater fish
| Autor: | Roberto D.V.S. Morais, Flavia S Lemos, Elizete Rizzo, Camila Ferreira Sales, Hélio Batista dos Santos, Ralph Gruppi Thomé, Nilo Bazzoli, Ana Paula Barbosa Pinheiro |
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| Rok vydání: | 2018 |
| Předmět: |
Hot Temperature
Embryonic Development Caspase 3 Apoptosis Reproductive technology Biology Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Heat shock protein Genetics Animals HSP70 Heat-Shock Proteins Molecular Biology 030304 developmental biology 0303 health sciences 030219 obstetrics & reproductive medicine TUNEL assay Embryogenesis Embryo Hsp70 Blastocyst Reproductive Medicine Animal Science and Zoology Characiformes Heat-Shock Response Developmental Biology Biotechnology |
| Zdroj: | Reproduction, fertility, and development. 31(3) |
| ISSN: | 1031-3613 |
| Popis: | Fish embryos are particularly vulnerable to temperature changes, with the effects varying with developmental stage. The major aim of the present study was to analyse the relationship between apoptosis and heat shock protein (HSP) 70 during embryo development under thermal stress conditions. To this end, Prochilodus lineatus embryos at the blastopore closure stage were subjected to one of three thermal treatments for 1h (Group 1, 25°C (control); Group 2, 20°C; Group 3, 30°C) and then examined at 0, 4 and 8h posttreatment (h.p.t.). The viability of embryos was highest in Group 1 (81.33±16.65%), followed by Group 3 and Group 2 (75.33±12.10% and 68.67±16.86% respectively), with significant difference between Groups 1 and 2 (P |
| Databáze: | OpenAIRE |
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