Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure
| Autor: | Dong Hoi Kim, Kunal Pal, Doman Kim, Sangyong Lim, Thi Thanh Hanh Nguyen, Ho-yong Jung, Taeyun Lim, Jae Wook Lee, Il-Kyoon Mok |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sucrose Antioxidant Glycosylation medicine.medical_treatment ved/biology.organism_classification_rank.species Hydrostatic pressure Bioengineering Leuconostoc mesenteroides 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Neuroprotection Antioxidants Dextransucrase Cell Line Crocin 03 medical and health sciences chemistry.chemical_compound Mice 010608 biotechnology Crocus sativus medicine Hydrostatic Pressure Animals Viability assay Food science biology Molecular Structure Chemistry ved/biology Water biology.organism_classification Carotenoids 030104 developmental biology Solubility Glucosyltransferases Biotechnology |
| Zdroj: | Enzyme and microbial technology. 140 |
| ISSN: | 1879-0909 |
| Popis: | Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation. In order to improve the functionality of crocin, α-glucosyl-(1→6)-trans-crocins (C-Gs) were synthesized using sucrose and dextransucrase from Leuconostoc mesenteroides. High hydrostatic pressure (HHP) technique was applied to the synthesis process of C-Gs in order to improve its transglucosylation yield. A 100 MPa HHP condition enhanced the production yield of C-Gs by 1.95 times compared to that of 0.1 MPa atmospheric pressure. Novel C-Gs were purified by HPLC, and their chemical structures were determined using NMR analysis. Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %). This advanced neuroprotection of novel C-Gs could be highly associated with their enhanced antioxidant activity. Thus, the enhanced water solubility and functionality of novel C-Gs can induce better clinical efficacy of neuroprotection than trans-crocin. |
| Databáze: | OpenAIRE |
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