Study of the correlation between MEIA and ELISA methods for FK 506 determination in liver transplant recipients
| Autor: | P. Rucay, E. Rudant, Y. Bezie, H. Bismuth, G. Fredj, L. Bonhomme-Faivre, N. Manuel |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Statistics as Topic Urology Enzyme-Linked Immunosorbent Assay Tacrolimus Nephrotoxicity Blood plasma medicine Humans Pharmacology (medical) Child Whole blood Aged Pharmacology Reproducibility Chemistry Liter Repeatability Middle Aged Liver Transplantation Transplantation Toxicity Immunology Female Immunosuppressive Agents |
| Zdroj: | Journal of clinical pharmacy and therapeutics. 22(2) |
| ISSN: | 0269-4727 |
| Popis: | SUMMARY Background and objectives: FK 506 is an immunosuppressive macrolide advocated for prevention of graft rejection. Plasma or blood FK 506 levels must be determined to strike a balance between FK 506 toxicity and graft rejection. The first aim of this study was to compare an automated microparticle enzyme immunosorbent assay (MEIA) method (on whole blood) with the reference enzyme-linked immunosorbent assay (ELISA) method (on plasma). A second aim was to compare the two methods for prediction of FK 506 nephrotoxicity. Patients and methods: Forty-seven patients were studied comprising 128 samples. All were treated with FK 506 on a compassionate basis. For each patient, the concentrations of FK 506 were determined in plasma by means of ELISA and in whole blood by MEIA. Results: The repeatability and the reproducibility of these two methods were similar. The inter-patient correlation coefficient between MEIA and ELISA, determined on 128 samples from 47 liver recipients, was satisfactory (r=0.82). From these 47 patients, the intra-patient correlation coefficients were calculated for 17 of them. The intra-patient correlation coefficients were between 0.63 and 0.98 for 15 patients, and between 0.26 and 0.55 in the remaining two cases. Mean creatinine plasma levels in the 55 samples below the median FK 506 value in the MEIA method and in the 55 with values above the median (120 and 134 μmol/litre, respectively) were significantly different (P |
| Databáze: | OpenAIRE |
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