AKAP9 Is Essential for Spermatogenesis and Sertoli Cell Maturation in Mice
| Autor: | Suzanne Hartford, Kerry J. Schimenti, Laurie B. Griffin, John C. Schimenti, Sky K. Feuer, Janice K. Pendola, Carl Lessard, Claire A. Bovet, Nancy R. Graham, Jeremy O. Ward |
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| Rok vydání: | 2013 |
| Předmět: |
Anti-Mullerian Hormone
Male Somatic cell A Kinase Anchor Proteins Spindle Apparatus Investigations Tight Junctions Mice Genetics medicine Animals Spermatogenesis Blood–testis barrier Sertoli Cells Tight junction biology Gap Junctions Anti-Müllerian hormone Sertoli cell Spermatozoa Mice Mutant Strains Spindle apparatus Cell biology Meiosis Protein Transport medicine.anatomical_structure Thyroid hormone receptor alpha Organ Specificity Connexin 43 Zonula Occludens-1 Protein biology.protein Microtubule-Associated Proteins Cyclin-Dependent Kinase Inhibitor p27 Thyroid Hormone Receptors alpha |
| Zdroj: | Genetics. 194:447-457 |
| ISSN: | 1943-2631 |
| Popis: | Mammalian male fertility relies on complex inter- and intracellular signaling during spermatogenesis. Here we describe three alleles of the widely expressed A-kinase anchoring protein 9 (Akap9) gene, all of which cause gametogenic failure and infertility in the absence of marked somatic phenotypes. Akap9 disruption does not affect spindle nucleation or progression of prophase I of meiosis but does inhibit maturation of Sertoli cells, which continue to express the immaturity markers anti-Mullerian hormone and thyroid hormone receptor alpha in adults and fail to express the maturation marker p27Kip1. Furthermore, gap and tight junctions essential for blood–testis barrier (BTB) organization are disrupted. Connexin43 (Cx43) and zona occludens-1 are improperly localized in Akap9 mutant testes, and Cx43 fails to compartmentalize germ cells near the BTB. These results identify and support a novel reproductive tissue-specific role for Akap9 in the coordinated regulation of Sertoli cells in the testis. |
| Databáze: | OpenAIRE |
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