Green tea catechins alleviate autoimmune symptoms and visual impairment in a murine model for human chronic intraocular inflammation by inhibiting Th17-associated pro-inflammatory gene expression
| Autor: | Kai On Chu, Yolanda W. Y. Yip, Chi Pui Pang, Tsz Kin Ng, Wing Ki Hui, Sun-On Chan, Jian Li, Jing Na He, Qiu Xiao Yu, Jialin Ren, Wai Kit Chu |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Vision Disorders lcsh:Medicine Green tea extract Pharmacology Article Catechin Uveitis Mice 03 medical and health sciences 0302 clinical medicine Immunity Oral administration Electroretinography medicine Animals lcsh:Science Dexamethasone Inflammation Microscopy Confocal Multidisciplinary Tea medicine.diagnostic_test Interleukin-6 Tumor Necrosis Factor-alpha business.industry Interleukin-17 lcsh:R Therapeutic effect medicine.disease eye diseases Disease Models Animal 030104 developmental biology Th17 Cells lcsh:Q Tumor necrosis factor alpha sense organs business Tomography Optical Coherence 030217 neurology & neurosurgery Papilledema medicine.drug |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-9 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Autoimmune uveitis is a sight-threatening disease mainly caused by dysregulation of immunity. We investigated the therapeutic effects of green tea extract (GTE) and its major component, epigallocatechin-3-gallate (EGCG), on a murine model of experimental autoimmune uveoretinitis (EAU). Oral administration of GTE, EGCG, dexamethasone, or water, which started 5 days before the induction, was fed every two days to each group. On day 21 post induction, the eyes were examined by confocal scanning laser ophthalmoscopy, optical coherence tomography (OCT), fundus fluorescein angiography (FFA) and electroretinography (ERG) prior to sacrificing the animals for histological assessments and gene expression studies. Retinal-choroidal thicknesses (RCT) and major retinal vessel diameter were measured on OCT sections and FFA images, respectively. Comparing to water-treated EAU animals, GTE attenuated uveitis clinical manifestations, RCT increase (1.100 ± 0.013 times vs 1.005 ± 0.012 times, P P IL-1β), IL-6, IL-17A, and tumor necrosis factor alpha (TNF-α)] expressions were down regulated by GTE and EGCG treatments, which showed no detectable morphological defects in liver and kidney in non-induced and EAU mice. Our findings suggest that GTE consumption can serve as a potent therapeutic agent as well as a food supplement for developing alternative treatments against autoimmune uveitis. |
| Databáze: | OpenAIRE |
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