Histological analysis of induced membranes in patients whose bone defects were treated with the Masquelet technique to identify factors affecting the vascularity of induced membranes
Autor: | Keisuke Oe, Tomoyuki Matsumoto, Masato Komatsu, Naoe Jimbo, Tomoo Itoh, Ryosuke Kuroda, Takehiko Matsushita, Yoshitada Sakai, Tomoaki Fukui, Takahiro Niikura, Shinya Hayashi |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Adult
Male medicine.medical_specialty Histology Vascularity Diseases of the musculoskeletal system Bone and Bones 03 medical and health sciences Young Adult 0302 clinical medicine Fibrosis Diabetes mellitus medicine Humans Orthopedics and Sports Medicine Femur Orthopedic Procedures 030212 general & internal medicine Tibia Aged Retrospective Studies Orthopedic surgery 030222 orthopedics Bone Transplantation business.industry Masquelet technique Induced membrane Bone Cements Bone defect Middle Aged Plastic Surgery Procedures medicine.disease Surgery medicine.anatomical_structure RC925-935 Female medicine.symptom business RD701-811 Blood vessel Research Article |
Zdroj: | Journal of Orthopaedic Surgery and Research Journal of Orthopaedic Surgery and Research, Vol 16, Iss 1, Pp 1-9 (2021) |
ISSN: | 1749-799X |
Popis: | Background Rich vascularity of the induced membrane (IM) is important for Masquelet reconstruction surgery. The factors affecting IM vascularity are not completely understood. This study aimed to investigate these factors using histological samples of human IMs. Methods We retrospectively evaluated 36 patients whose bone defects were treated using the Masquelet technique. Two clinical pathologists analyzed histological sections of IM pieces (1 cm2). The number of blood vessels per 1 mm2 was counted and compared among men and women, femur or tibia, with and without free flap surgery, antibiotic impregnation to the cement, osteogenesis inside the membrane, smoking, and diabetes mellitus. The number of blood vessels within the same patient was compared among different time points. Correlation analysis was performed among blood vessel numbers and patient age, duration of cement spacer placement, and histological grading scales (inflammation, foreign body reaction, and fibrosis). Results IM formation with rich vascularity and some inflammation, foreign body reaction, and fibrosis were histologically confirmed in all patients. We found 37.4 ± 19.1 blood vessels per 1 mm2. The number of blood vessels was significantly lower in patients with than in those without free flap surgery; it was higher in patients with osteogenesis inside the IM. No significant correlations were found in any of the analyses. Conclusion Sex, patient age, smoking, diabetes mellitus, femur or tibia, duration of cement spacer placement, and antibiotic impregnation to the cement did not affect IM vascularization. IM vascularization was reduced in patients with than in those without free flap surgery. |
Databáze: | OpenAIRE |
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