Evaluation of methods of measuring glomerular and nutrient blood flow in rat kidneys
| Autor: | W. E. Yarger, N. W. Schrader, M. A. Boyd |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
medicine.medical_specialty
Physiology Inulin Renal function Kidney Microsphere law.invention chemistry.chemical_compound Nutrient law Physiology (medical) Cortex (anatomy) Internal medicine Methods medicine Animals Filtration Chromatography Chemistry Blood flow Rubidium Microspheres Rats medicine.anatomical_structure Endocrinology Regional Blood Flow Female p-Aminohippuric Acid Cardiology and Cardiovascular Medicine Glomerular Filtration Rate |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 235:H592-H600 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1978.235.5.h592 |
| Popis: | In normal rats, glomerular plasma flow rates (GPF) were estimated from the uptake of microspheres, and single-nephron filtration rates were estimated by Hanssen's technique in order to calculate single-nephron filtration fractions (SNFF) for outer (C1), middle, (C2), and deep (C3) nephrons. With large microspheres (15 micron), SNFF averaged 0.19, 0.41, and 0.63, and with small microspheres (9 micron), SNFF averaged 0.25, 0.48, and 0.42 for areas C1, C2, and C3, respectively. Kidney filtration fractions (FF) averaged 0.36. Because microsphere experiments in normal rats have generally suggested that SNFF-C1 and FF are similar, we conclude that both types of microspheres overestimated outer cortical plasma flow rates, and probably underestimated inner cortical plasma flow rates. In addition, not all of the smaller microspheres were trapped in the glomeruli. Nutrient blood flow rates were estimated from the uptake of 86Rb. Values ranged from 7.3 ml/g per min in the outer cortex to 4.7 ml/g per min in the inner cortex. Because these values are very similar to measurements made by several other techniques, we conclude that 86Rb uptake adequately estimates cortical blood flow. Medullary blood flow estimates, however, increased with time and were probably too high. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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