Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories
| Autor: | Roman Pogranichniy, Kathy L. Toohey-Kurth, Donna M. Mulrooney, Laura B. Goodman, Steve Bolin, Roger K. Maes, Beate Crossley, Mary Lea Killian, Janice C. Pedersen, Rebecca L. Tallmadge, Mangkey A. Bounpheng, Susanne Hinkley |
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| Rok vydání: | 2020 |
| Předmět: |
Quality Control
Veterinary medicine practice guidelines General Veterinary business.industry AAVLD Best practice Real-Time Polymerase Chain Reaction Sensitivity and Specificity qPCR Focus Issue Real-time polymerase chain reaction Animals Medicine Veterinary Sciences real-time PCR Laboratories business Zoology |
| Zdroj: | J Vet Diagn Invest Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, vol 32, iss 6 |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter experts was convened to develop a set of best practices for performance of nucleic acid amplification assays. Consensus advice for the performance of preanalytical, analytical, and postanalytical steps is presented here, along with a review of supporting literature. |
| Databáze: | OpenAIRE |
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