Ruthenium Dendrimers against Human Lymphoblastic Leukemia 1301 Cells

Autor: Maksim Ionov, Francisco Javier de la Mata, Natalia Sanz del Olmo, Marta Denel, Dzmitry Shcharbin, Paula Ortega, Sylwia Michlewska, Aneta Rogalska, Maria Bryszewska, Damian Jacenik, Aleksandra Szwed
Přispěvatelé: Laboratory of Microscopic Imaging & Specialized Biological Techniques, Faculty of Biology & Environmental Protection, University of Lodz, 90-237 Lodz, Poland, Department of General Biophysics, Faculty of Biology & Environmental Protection, University of Lodz, 90-236 Lodz, Poland, Department of Medical Biophysics, Faculty of Biology & Protection, University of Lodz, 90-236 Lodz, Poland, Networking Research Center on Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN), 28029 Madrid, Spain, Department of Organic Chemistry and Inorganic Chemistry, Research Institute of Chemistry 'Andrés M. del Rio (IQAR)', Institute 'Ramón y Cajal' for Health Research (IRYCIS), University of Alcalá, 28805 Madrid, Spain, Department of Cytobiochemistry, Faculty of Biology & Protection, University of Lodz, 90-236 Lodz, Poland, Institute of Biophysics & Cell Engineering of NASB, 220072 Minsk, Belarus
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Apoptosis
02 engineering and technology
lcsh:Chemistry
Cytotoxicity
lcsh:QH301-705.5
Spectroscopy
chemistry.chemical_classification
Membrane Potential
Mitochondrial
Gene Expression Regulation
Leukemic
General Medicine
Precursor Cell Lymphoblastic Leukemia-Lymphoma
021001 nanoscience & nanotechnology
Computer Science Applications
Ruthenium
Leukemia
Real-time polymerase chain reaction
Drug delivery
cytotoxicity
Comet Assay
0210 nano-technology
Dendrimers
Cell Survival
chemistry.chemical_element
Antineoplastic Agents
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Microscopy
Electron
Transmission
Dendrimer
carbosilane dendrimer
Cell Line
Tumor
medicine
Humans
Physical and Theoretical Chemistry
ruthenium
Molecular Biology
Reactive oxygen species
Organic Chemistry
medicine.disease
Comet assay
030104 developmental biology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
drug delivery
Biophysics
1301 human lymphoblastic leukemia
Reactive Oxygen Species
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 21, Iss 4119, p 4119 (2020)
Volume 21
Issue 11
ISSN: 1422-0067
Popis: Ruthenium atoms located in the surfaces of carbosilane dendrimers markedly increase their anti-tumor properties. Carbosilane dendrimers have been widely studied as carriers of drugs and genes owing to such characteristic features as monodispersity, stability, and multivalence. The presence of ruthenium in the dendrimer structure enhances their successful use in anti-cancer therapy. In this paper, the activity of dendrimers of generation 1 and 2 against 1301 cells was evaluated using Transmission Electron Microscopy, comet assay and Real Time PCR techniques. Additionally, the level of reactive oxygen species (ROS) and changes of mitochondrial potential values were assessed. The results of the present study show that ruthenium dendrimers significantly decrease the viability of leukemia cells (1301) but show low toxicity to non-cancer cells (peripheral blood mononuclear cells&mdash
PBMCs). The in vitro test results indicate that the dendrimers injure the 1301 leukemia cells via the apoptosis pathway.
Databáze: OpenAIRE
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