Transcriptome dataset of trunk neural crest cells migrating along the ventral pathway of chick embryos
| Autor: | Felipe Monteleone Vieceli, Christina Murko, Marianne E. Bronner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
education.field_of_study Multidisciplinary animal structures Population Neural crest Embryo In situ hybridization Biology lcsh:Computer applications to medicine. Medical informatics Trunk Cell biology Transcriptome 03 medical and health sciences 030104 developmental biology Biochemistry Genetics and Molecular Biology embryonic structures lcsh:R858-859.7 education Enhancer lcsh:Science (General) Illumina dye sequencing lcsh:Q1-390 |
| Zdroj: | Data in Brief Data in Brief, Vol 21, Iss, Pp 2547-2553 (2018) |
| ISSN: | 2352-3409 |
| Popis: | We present a transcriptome dataset generated from migratory chick trunk neural crest cells, which are destined to form components of the peripheral nervous system. Using the Sox10E1 enhancer, which specifically labels neural crest cells migrating on the trunk ventral pathway, we performed fluorescence activated cell sorting (FACS) of electroporated embryos to obtain a pure population of these cells for library preparation and Illumina sequencing. The results provide a list of genes that are enriched in the trunk neural crest. To validate the data, we performed in situ hybridization to visualize expression of selected transcripts. |
| Databáze: | OpenAIRE |
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