A Novel Immunoassay for Malondialdehyde-Conjugated Low-Density Lipoprotein Measures Dynamic Changes in the Blood of Patients Undergoing Coronary Artery Bypass Graft Surgery
| Autor: | Jon Anderson, Ameer Hamid Ahmed Khan, Adam Hartley, Steven Lynham, Bao Anh Vu Nguyen, Samata S. Pandey, Ramzi Khamis, Dorian O. Haskard, Michael M. Johns, Tareq Ammari, Mikhail Caga-Anan, Chiari Kojima |
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| Přispěvatelé: | Wellcome Trust, British Heart Foundation, National Institute for Health Research, Imperial Health Charity, Imperial College Healthcare NHS Trust- BRC Funding, Imperial College Healthcare NHS Trust |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
malondialdehyde
medicine.medical_specialty Biochemistry & Molecular Biology Physiology Clinical Biochemistry Inflammation Chemistry Medicinal RM1-950 medicine.disease_cause Biochemistry Article CIRCULATING OXIDIZED LDL chemistry.chemical_compound medicine low-density lipoprotein (LDL) antibodies Pharmacology & Pharmacy Molecular Biology Science & Technology medicine.diagnostic_test business.industry Cell Biology anti-oxidized LDL antibodies assay Malondialdehyde Surgery medicine.anatomical_structure chemistry oxidized LDL inflammation Low-density lipoprotein Immunoassay Food Science & Technology MARKER lipids (amino acids peptides and proteins) Therapeutics. Pharmacology medicine.symptom atherosclerosis business Life Sciences & Biomedicine Homeostasis Oxidative stress Artery Lipoprotein |
| Zdroj: | Antioxidants Volume 10 Issue 8 Antioxidants, Vol 10, Iss 1298, p 1298 (2021) |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox10081298 |
| Popis: | Oxidized low-density lipoproteins play an important role in tissue pathology. In this study, we report a sensitive novel enzyme-linked immunosorbent assay for the detection of malondialdehyde-modified low-density lipoprotein (MDA-LDL), a key component of oxidized LDL. The assay is capable of measuring a variable presence of MDA-LDL within human plasma and serum. We demonstrate the robust nature of the assay on samples stored for over 20 months, as well as high inter-operator reproducibility (r = 0.74, p < 0.0001). The assay was capable of detecting dynamic changes in patient blood samples after coronary artery bypass graft surgery, indicating synthesis or release of MDA-LDL with the oxidative stress of surgery, followed by homeostatic clearance. This robust, sensitive and specific assay for circulating MDA-LDL will serve as a valuable translational tool for the improved detection of oxidative forms of LDL in response to a range of physiological or pathological stimuli, with potential clinical applicability. |
| Databáze: | OpenAIRE |
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