Differentiation of human dendritic cell subsets for immune tolerance induction
| Autor: | A Héraud, V Chabot, F Dehaut, Nora Deluce-Kakwata-Nkor, F Halary, L Lamendour, Florence Velge-Roussel, Zoran Ivanovic |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult CD14 Cellular differentiation Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Receptors Cell Surface Biology Peripheral blood mononuclear cell Monocytes Immune tolerance 03 medical and health sciences 0302 clinical medicine Immune system Antigens CD Immune Tolerance Humans Lectins C-Type Interleukin 4 Cells Cultured Immunomagnetic Separation Biochemistry (medical) Granulocyte-Macrophage Colony-Stimulating Factor hemic and immune systems Cell Differentiation Hematology Dendritic cell Dendritic Cells Flow Cytometry Interleukin-10 Cytapheresis Interleukin 10 030104 developmental biology Immunology Interleukin-4 Cell Adhesion Molecules 030215 immunology |
| Zdroj: | Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine. 25(1) |
| ISSN: | 1953-8022 |
| Popis: | Objectives Since no further progress was achieved, in order to improve the long-term organ transplantation outcome, the immune tolerance appears as an interesting therapeutic goal. Dendritic cells (DCs) are specialized cells participating in the homeostasis of the immune response. Moreover, subsets of DCs, identified in humans, appear to have their respective competences in immune response modulation. Our objective is to purify from PBMC or to differentiate DC subsets from monocytes using several strategies and evaluate their IL10 secretion. Methods CD14 + cells were purified from peripheral blood mononuclear cell (PBMC) by affinity beads and cultured with cytokines up to 7 days. The pDCs were purified with anti-BDCA-2 beads from PBMC fraction enriched by Percoll ® gradient. The moDCs, pDCs and moLCs subsets were analyzed by phenotype labelling and FACS analyses and IL10 secretion measured by ELISA. Results The moDCs were characterized by the CD209 expression and a lower expression of CD1a markers. Expression of CD207 and CD1a markers characterized moLCs and CD123 + /BDCA-2 + pDCs. Variable IL-10 secretions were shown between the three DC subsets, both at basal and activated levels. Conclusions As the several DC populations studied have different capacities of IL-10 synthesis, they might play, among others, distinct roles in the induction of immune tolerance. |
| Databáze: | OpenAIRE |
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