Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer
| Autor: | Paul D R Johnson, Till F. Omansen, Jason K. Axford, Sacha J. Pidot, Jessica L. Porter, Renee Marcsisin, Timothy P. Stinear, Kirstie M. Mangas, Brian O. Howden, Weiguang Zeng, John R. Wallace |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Bacterial Diseases
0301 basic medicine Buruli ulcer SOUTHEASTERN AUSTRALIA Disease Vectors Skin infection Mosquitoes DISEASE Geographical Locations chemistry.chemical_compound 0302 clinical medicine NAUCORIS-CIMICOIDES Aedes MYCOLACTONE Medicine and Health Sciences BENIN EPIDEMIOLOGY Needlestick Injuries Mycolactone Buruli Ulcer Mice Inbred BALB C biology Infectious dose lcsh:Public aspects of medicine Animal Models 3. Good health Actinobacteria Insects Infectious Diseases GHANA Experimental Organism Systems Mycobacterium ulcerans Female Research Article Neglected Tropical Diseases Skin Infections lcsh:Arctic medicine. Tropical medicine Arthropoda lcsh:RC955-962 Oceania 030231 tropical medicine Mouse Models Dermatology Aedes aegypti Aedes Aegypti Research and Analysis Methods Insect bites and stings Microbiology 03 medical and health sciences Model Organisms medicine Animals Bacteria IDENTIFICATION Organisms Australia Public Health Environmental and Occupational Health Biology and Life Sciences Insect Bites and Stings lcsh:RA1-1270 Tropical Diseases biology.organism_classification medicine.disease Invertebrates ENVIRONMENTAL-SAMPLES Insect Vectors Species Interactions 030104 developmental biology chemistry People and Places RISK-FACTORS |
| Zdroj: | PLoS Neglected Tropical Diseases, Vol 11, Iss 4, p e0005553 (2017) PLoS Neglected Tropical Diseases, 11(4). PUBLIC LIBRARY SCIENCE PLoS Neglected Tropical Diseases |
| ISSN: | 1935-2735 1935-2727 |
| Popis: | Addressing the transmission enigma of the neglected disease Buruli ulcer (BU) is a World Health Organization priority. In Australia, we have observed an association between mosquitoes harboring the causative agent, Mycobacterium ulcerans, and BU. Here we tested a contaminated skin model of BU transmission by dipping the tails from healthy mice in cultures of the causative agent, Mycobacterium ulcerans. Tails were exposed to mosquito (Aedes notoscriptus and Aedes aegypti) blood feeding or punctured with sterile needles. Two of 12 of mice with M. ulcerans contaminated tails exposed to feeding A. notoscriptus mosquitoes developed BU. There were no mice exposed to A. aegypti that developed BU. Eighty-eight percent of mice (21/24) subjected to contaminated tail needle puncture developed BU. Mouse tails coated only in bacteria did not develop disease. A median incubation time of 12 weeks, consistent with data from human infections, was noted. We then specifically tested the M. ulcerans infectious dose-50 (ID50) in this contaminated skin surface infection model with needle puncture and observed an ID50 of 2.6 colony-forming units. We have uncovered a biologically plausible mechanical transmission mode of BU via natural or anthropogenic skin punctures. Author summary Buruli ulcer is a neglected tropical disease caused by infection with Mycobacterium ulcerans. Unfortunately, how people contract this disease is not well understood. Here we show for the first time using experimental infections in mice that a very low dose of M. ulcerans delivered beneath the skin by a minor injury caused by a blood-feeding insect (mosquito) or an experimental needle puncture is sufficient to cause Buruli ulcer. This research provides important laboratory evidence to advance our understanding of Buruli ulcer disease transmission. |
| Databáze: | OpenAIRE |
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