Calcium ion-dependent entry of the membrane-containing bacteriophage PM2 into its Pseudoalteromonas host

Autor: Dennis H. Bamford, Mart Krupovic, Virginija Cvirkaitė-Krupovič, Rimantas Daugelavičius
Přispěvatelé: University of Helsinki, Vilnius University [Vilnius], Vytautas Magnus University - Vytauto Didziojo Universitetas (VDU), This work was supported by the Finnish Center of Excellence Program (2006–2011) of the Academy of Finland (Grant 1129684 to DHB) and the Viikki Graduate School in Molecular Biosciences (MK and VC-K)., The skilful technical assistance of Petri Papponen and Terhi Kemppinen is greatly acknowledged.
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Time Factors
Lysis
membrane-containing bacteriophage
MESH: Corticoviridae
MESH: Virus Internalization
[SDV]Life Sciences [q-bio]
chemistry.chemical_element
Receptors
Cell Surface
Ca2+-dependent virus entry
Calcium
complex mixtures
Divalent
03 medical and health sciences
Adenosine Triphosphate
Pseudoalteromonas
Virology
Host outer membrane
MESH: Adenosine Triphosphate
030304 developmental biology
MESH: Receptors
Cell Surface
chemistry.chemical_classification
bacteriophage PM2
0303 health sciences
biology
030306 microbiology
MESH: Pseudoalteromonas
Cell Membrane
Corticoviridae
MESH: Time Factors
Virus Internalization
biology.organism_classification
Molecular biology
Membrane
chemistry
Cytoplasm
MESH: Calcium
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Biophysics
cell lysis
Intracellular
MESH: Cell Membrane
Zdroj: Virology
Virology, Elsevier, 2010, 405 (1), pp.120-128. ⟨10.1016/j.virol.2010.05.021⟩
ISSN: 0042-6822
1096-0341
DOI: 10.1016/j.virol.2010.05.021⟩
Popis: International audience; Marine bacteriophage PM2 infects gram-negative Pseudoalteromonas species and is currently the only assigned member of the Corticoviridae family. The icosahedral protein shell covers an internal protein-rich phage membrane that encloses the highly supercoiled dsDNA genome. In this study we investigated PM2 entry into the host. Our results indicate that PM2 adsorption to the host is dependent on the intracellular ATP concentration, while genome penetration through the cytoplasmic membrane depends on the presence of millimolar concentrations of calcium ions in the medium. In the absence of Ca(2+) the infection is arrested at the entry stage but can be rescued by the addition of Ca(2+). Interestingly, PM2 entry induces abrupt cell lysis if the host outer membrane is not stabilized by divalent cations. Experimental data described in this study in combination with results obtained previously allowed us to propose a sequential model describing the entry of bacteriophage PM2 into the host cells.
Databáze: OpenAIRE
Externí odkaz: