A micronucleus assay in sea urchin embryos
| Autor: | Kyoko Saotome, Makoto Hayashi, Toshio Sofuni |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
animal structures Mitomycin Health Toxicology and Mutagenesis Cell Count Mutagen Vinblastine medicine.disease_cause Embryonic and Fetal Development Hemicentrotus chemistry.chemical_compound Botany Genetics medicine Animals Micronucleus Tests Dose-Response Relationship Drug Staining and Labeling biology Mitomycin C Acridine orange Cytarabine Embryo Blastula biology.organism_classification Molecular biology Acridine Orange chemistry Sea Urchins embryonic structures Micronucleus test Urea Female Environmental Monitoring Mutagens |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 446:121-127 |
| ISSN: | 1383-5718 |
| DOI: | 10.1016/s1383-5718(99)00155-2 |
| Popis: | We have developed a micronucleus assay for use in sea urchin embryos. The embryos at the early blastula stage (about 256 cells) were exposed to genotoxic chemicals overnight until control embryos have reached the gastrula stage. Then all embryos were suspended in 1 M urea, dissociated by pipetting, and fixed with methanol:acetic acid (9:1). The preparations were air-dried and stained with acridine orange. The test chemicals (mitomycin C [MMC], vinblastine and 1-beta-D-arabinofuranosylcytosine [Ara C]) induced clear micronuclei dose-dependently. The maximum frequency induced with MMC was 2-3% in Clypeaster japonicus and 1-2% in Hemicentrotus pulcherrimus. |
| Databáze: | OpenAIRE |
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