Plasma Viral miRNAs Indicate a High Prevalence of Occult Viral Infections

Autor: Stefan Tudor, Meng Chen, Jeffrey J. Tarrand, Dana Elena Giza, Cristina Ivan, Maria Ciccone, Enrique Fuentes-Mattei, Michael J. Keating, Xinna Zhang, Osman Aykan Kargin, Florea Lupu, Nayra Soares do Amaral, George A. Calin, Catalin Vasilescu, Alessandra Ferrajoli, Masayoshi Shimizu, Pilar Mur, Sai Ching J. Yeung, John T. Manning
Přispěvatelé: Halk Sağlığı
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Micro RNAs
Chronic lymphocytic leukemia
viruses
lcsh:Medicine
Antibodies
Viral
medicine.disease_cause
Immunoglobulin G
Serology
Cohort Studies
Leukocyte Count
HHV4
Prevalence
HHV8
In Situ Hybridization
lcsh:R5-920
virus diseases
General Medicine
Viral Load
3. Good health
Real-time polymerase chain reaction
Virus Diseases
Herpesvirus 8
Human
RNA
Viral
lcsh:Medicine (General)
Viral load
Research Paper
Herpesviruses
ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION
Enzyme-Linked Immunosorbent Assay
KSHV
Biology
Real-Time Polymerase Chain Reaction
Viral miRNAs
General Biochemistry
Genetics and Molecular Biology
Virus
03 medical and health sciences
Antigen
EBV
medicine
Humans
Lymphocyte Count
ComputingMethodologies_COMPUTERGRAPHICS
Infection prevalence
lcsh:R
Reproducibility of Results
Herpesvirus
medicine.disease
Epstein–Barr virus
Virology
MicroRNAs
030104 developmental biology
Immunology
biology.protein
Zdroj: EBioMedicine, Vol 20, Iss C, Pp 182-192 (2017)
Recercat. Dipósit de la Recerca de Catalunya
instname
Dipòsit Digital de la UB
Universidad de Barcelona
EBioMedicine
ISSN: 2352-3964
Popis: Graphical abstract Image 1
Prevalence of Kaposi sarcoma-associated herpesvirus (KSHV/HHV-8) varies greatly in different populations. We hypothesized that the actual prevalence of KSHV/HHV8 infection in humans is underestimated by the currently available serological tests. We analyzed four independent patient cohorts with post-surgical or post-chemotherapy sepsis, chronic lymphocytic leukemia and post-surgical patients with abdominal surgical interventions. Levels of specific KSHV-encoded miRNAs were measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and KSHV/HHV-8 IgG were measured by immunoassay. We also measured specific miRNAs from Epstein Barr Virus (EBV), a virus closely related to KSHV/HHV-8, and determined the EBV serological status by ELISA for Epstein-Barr nuclear antigen 1 (EBNA-1) IgG. Finally, we identified the viral miRNAs by in situ hybridization (ISH) in bone marrow cells. In training/validation settings using independent multi-institutional cohorts of 300 plasma samples, we identified in 78.50% of the samples detectable expression of at least one of the three tested KSHV-miRNAs by RT-qPCR, while only 27.57% of samples were found to be seropositive for KSHV/HHV-8 IgG (P
Highlights • There is no agreement on a standard assay to detect the true prevalence of Kaposi sarcoma-associated herpesvirus (KSHV) infection. • Measurement of the viral miRNAs in plasma by RT-qPCR allows a direct and accurate assessment of viral infection. • Measurement of the viral miRNAs in plasma by RT-qPCR shows prevalence of KSHV infection in immuno-depressed patients. • Measurement of plasma viral miRNAs for viral infection assessment has the potential to become a “gold” standard method in the clinical practice. Chronic viral infections represent risk factors for diseases and development of infection-related complications. There is no agreement on a standard assay to detect the true prevalence of Kaposi sarcoma-associated herpesvirus (KSHV) infection. The current method used in the clinical practice (ELISA-test) identifies a great geographic variation in KSHV seroprevalence and may underestimate the true-prevalence of KSHV infection. Here we showed that detection of plasma viral miRNAs levels for the identification of viral infection (e.g., KSHV, Epstein-Bar virus or EBV) is more accurate than the current method for detection of virus-derived antigen, especially in patients with low number of immune cells.
Databáze: OpenAIRE
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