The effect of 6-dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes

Autor:	RM Moor, Pasqualino Loi, Jr J Fulka, Luisa Bogliolo, Sergio Ledda
Rok vydání:	1996
Předmět:	DNA Replication Mice Inbred ICR Sheep Ethanol DNA synthesis Metaphase ii Adenine DNA replication Context (language use) Cell Biology Cell biology Mice chemistry.chemical_compound Species Specificity chemistry Oocytes Animals Cattle Female Cells Cultured Developmental Biology
Zdroj:	Zygote. 4:7-9
ISSN:	1469-8730 0967-1994
DOI:	10.1017/s0967199400002835
Popis:	SummaryMouse, sheep and bovine metaphase II oocytes were briefly preincubated in medium with 6-dimethyl-aminopurine (6-DMAP) and thereafter activated with ethanol before further culture in 6-DMAP supplemented medium. The presence of 6-DMAP enhances the efficiency of activation and the speed of pronuclear formation but has no effect on DNA synthesis. These results are discussed in the context of recently published data showing that the preincubation of condensed chromosomes with 6-DMAP blocks DNA synthesis when nuclei become reformed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79eca4e09f72796289332a500accb166 https://doi.org/10.1017/s0967199400002835 Zobrazit plný text záznamu