Enhancing Tissue Adhesion and Osteoblastic Differentiation of MC3T3‐E1 Cells on Poly(aryl ether ketone) by Chemically Anchored Hydroxyapatite Nanocomposite Hydrogel Coating
| Autor: | Wentao Liu, Xigao Jian, Xitong Cheng, Cheng Liu, Liang Pan, Yizheng Li, Chengde Liu |
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| Rok vydání: | 2021 |
| Předmět: |
Polymers and Plastics
Swine Nanogels Tissue Adhesions Bioengineering Ether 02 engineering and technology 010402 general chemistry 01 natural sciences Osseointegration Biomaterials chemistry.chemical_compound Osteogenesis Materials Chemistry Animals Bifunctional Acrylic acid Tissue Adhesion Nanocomposite Chemistry technology industry and agriculture Ketones 021001 nanoscience & nanotechnology 0104 chemical sciences Durapatite Chemical engineering Attenuated total reflection Surface modification 0210 nano-technology Biotechnology |
| Zdroj: | Macromolecular Bioscience. 21:2100078 |
| ISSN: | 1616-5195 1616-5187 |
| Popis: | Tissue adhesion to bone implant and osteoblastic differentiation are the key factors to achieve poly(aryl ether ketone) (PAEK) implant osseointegration. However, physical interaction of implant with tissue and hydroxyapatite coating suffers from slow implant tissue integration and lack of long-term stability. In this study, a novel poly(phthalazinone ether sulfone ketone) containing allyl groups (APPBAESK) is coated onto PPBESK sheet for reacting with the allyl groups of the hydrogel coating to enhance its stability. N-Succinimidyl (NHS)-ester activated group and nano-hydroxyapatite (nano-HA) are introduced into the hydrogel synthesized from gelatin methacrylate (GelMA) and acrylic acid to construct a nanocomposite hydrogel coating on PPBESK which is a promising PAEK implant material. The hydrophilicity of the PPBESK sheet is improved by the hydrogel coating. The chemical components of the nanocomposite hydrogel coating are confirmed by X-ray photoelectron spectroscope, Attenuated total reflection infrared, and X-ray powder diffraction. The tissue shear adhesion strength of the hydrogel coating toward pig skin is enhanced due to the synergism of NHS-ester activated group and nano-HA. The osteogenic differentiation of MC3T3-E1 preosteoblasts is promoted by nano-HA in nanocomposite hydrogel coating. Therefore, the bifunctional nanocomposite hydrogel coating provides a great application prospect in the surface modification of PAEK implants in bone tissue engineering. |
| Databáze: | OpenAIRE |
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