Turnover of CD4+ and CD8+ T lymphocytes in HIV-1 infection as measured by Ki-67 antigen
| Autor: | Sabine Yerly, Luc Perrin, Nicolas Sachsenberg, Alan S. Perelson, Bernard Hirschel, Dominique Leduc, Gérard A. Schockmel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
CD4-Positive T-Lymphocytes
Adult HIV Infections/ immunology medicine.medical_specialty Ki-67 Antigen/ analysis T cell Immunology HIV Infections CD8-Positive T-Lymphocytes Lymphocyte Activation Article Flow cytometry 03 medical and health sciences 0302 clinical medicine Antigen T-Lymphocyte Subsets Internal medicine medicine Immunology and Allergy Doubling time Humans 030304 developmental biology ddc:616 0303 health sciences biology medicine.diagnostic_test Cell growth HIV-1/ immunology Cell Cycle Half-life Antigens CD45 Articles CD4-Positive T-Lymphocytes/ cytology Flow Cytometry Flow Cytometry/methods T-Lymphocyte Subsets/cytology CD4 Lymphocyte Count CD8-Positive T-Lymphocytes/ cytology Endocrinology medicine.anatomical_structure Ki-67 Antigen Ki-67 biology.protein HIV-1 Leukocyte Common Antigens CD8 030215 immunology Half-Life |
| Zdroj: | Journal of Experimental Medicine, Vol. 187, No 8 (1998) pp. 1295-1303 The Journal of Experimental Medicine |
| ISSN: | 0022-1007 |
| Popis: | We investigated CD4+ and CD8+ T cell turnover in both healthy and HIV-1–infected adults by measuring the nuclear antigen Ki-67 specific for cell proliferation. The mean growth fraction, corresponding to the expression of Ki-67, was 1.1% for CD4+ T cells and 1.0% in CD8+ T cells in healthy adults, and 6.5 and 4.3% in HIV-1–infected individuals, respectively. Analysis of CD45RA+ and CD45RO+ T cell subsets revealed a selective expansion of the CD8+ CD45RO+ subset in HIV-1–positive individuals. On the basis of the growth fraction, we derived the potential doubling time and the daily turnover of CD4+ and CD8+ T cells. In HIV-1–infected individuals, the mean potential doubling time of T cells was five times shorter than that of healthy adults. The mean daily turnover of CD4+ and CD8+ T cells in HIV-1–infected individuals was increased 2- and 6-fold, respectively, with more than 40-fold interindividual variation. In patients with |
| Databáze: | OpenAIRE |
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