The Effect of Different Immunization Cycles of a Recombinant Mucin1-Maltose-Binding Protein Vaccine on T Cell Responses to B16-MUC1 Melanoma in Mice

Autor: Mengyu Jiang, Jingjing Wang, Hongyue Zhou, Guixiang Tai, Yu Liu, Guomu Liu, Zenan Zhang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
immunization cycle
T-Lymphocytes
Melanoma
Experimental
lcsh:Chemistry
0302 clinical medicine
Tumor Microenvironment
Cytotoxic T cell
Cytotoxicity
lcsh:QH301-705.5
Spectroscopy
Vaccines
Synthetic
General Medicine
Up-Regulation
Computer Science Applications
medicine.anatomical_structure
antitumor vaccine
030220 oncology & carcinogenesis
Female
Antibody
T cell
chemical and pharmacologic phenomena
Biology
Maltose-Binding Proteins
Article
Catalysis
CpG 2006
Inorganic Chemistry
03 medical and health sciences
Immune system
medicine
Animals
Humans
Physical and Theoretical Chemistry
Molecular Biology
neoplasms
Cell Proliferation
Tumor microenvironment
Mucin-1
Organic Chemistry
biochemical phenomena
metabolism
and nutrition
Mice
Inbred C57BL
Disease Models
Animal
CTL
030104 developmental biology
Immunization
lcsh:Biology (General)
lcsh:QD1-999
Immunology
biology.protein
MUC1-MBP
Spleen
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 5810, p 5810 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 16
ISSN: 1661-6596
1422-0067
Popis: We explored the effect of a recombinant mucin1-maltose-binding protein vaccine, including immunization cycles of recombinant mucin1-maltose-binding protein (MUC1-MBP) and CpG 2006 on T cell responses to human MUC1-overexpressing mouse melanoma B16 cells (B16-MUC1) melanoma in mice. We found that the vaccine had a significant antitumor effect, with the most obvious tumor-suppressive effect being observed in mice immunized five times. After more than five immunizations, the tumor inhibition rate decreased from 81.67% (five immunizations) to 43.67% (eight immunizations). To study the possible mechanism, Mucin-1(MUC1)-specific antibodies, IFN-&gamma
secretion by lymphocytes, and cytotoxic T lymphocyte (CTL) cytotoxicity were measured by enzyme-linked immunosorbent assay (ELISA) and a real-time cell analyzer (RTCA). T cell subsets and immunosuppressive cells in the mouse spleen and tumor microenvironment were analyzed by FACS. These results showed that five immunizations activated MUC1-specific Th1 and CTL and reduced the ratio of myeloid-derived suppressor cells (MDSCs) and Th17 in mice more significantly than eight immunizations, indicating that excessive frequency of the immune cycle leads to the increased numbers of immunosuppressive cells and decreased numbers of immunostimulatory cells, thereby inhibiting antitumor immune activity. This data provide an experimental foundation for the clinical application of a recombinant MUC1-MBP vaccine.
Databáze: OpenAIRE
Externí odkaz: