A lymphocyte differentiation and activation antigen, CZ-1, that distinguishes between CD8+ and unstimulated CD4+ T lymphocytes
| Autor: | Raymond M. Welsh, Appel Michael C, Carey L. O'Donnell, Joseph Walter Maciaszek, Mauricio Vargas-Cortes, Kate S. Yurkunas |
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| Rok vydání: | 1992 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD4-Positive T-Lymphocytes CD8 Antigens T-Lymphocytes Immunology Mice Inbred Strains Thymus Gland Lymphocyte Activation Immunoenzyme Techniques Interleukin 21 Mice Antigen Immunology and Allergy Cytotoxic T cell Animals biology Lymphocyte differentiation Antibodies Monoclonal T lymphocyte Molecular biology Killer Cells Natural Thymocyte biology.protein Antibody CD8 Spleen |
| Zdroj: | European journal of immunology. 22(4) |
| ISSN: | 0014-2980 |
| Popis: | We report the generation and cellular reactivity of a novel rat IgM monoclonal antibody (mAb), CZ-1, made against mouse natural killer (NK) cells activated in vivo. mAb CZ-1 recognizes a molecule whose properties are consistent with that of a trypsin-sensitive, non-phosphatidyl inositol-linked sialoglycoprotein. The expression of the antigen recognized by mAb CZ-1 is restricted mostly to cells of the lymphoid lineage. The antigen is expressed on 10%-25% of bone marrow cells and 3%-5% of thymocytes. Analysis of thymocyte subpopulations indicates expression of the CZ-1 antigen on 100% of the NK1.1+, 27% of the CD4-CD8-, 1.1% of the CD4+CD8+, 1.1% of the CD4+CD8-, and 33% of the CD4-CD8+ cells. In the spleen, the CZ-1 antigen is expressed on B lymphocytes, NK cells, and virtually all CD8+ T lymphocytes. Most unstimulated CD4+ splenic T lymphocytes, monocytes and polymorphonuclear cells, with the notable exception of basophils, do not react with mAb CZ-1. CD4+ T cells activated in vivo by virus infection or in vitro by anti-CD3 and interleukin-2 express the CZ-1 antigen. These results indicate that mAb CZ-1 identifies a novel inducible lymphocyte activation/differentiation antigen that distinguishes between thymic and unstimulated splenic CD4+ and CD8+ T lymphocytes. This mAb will be a useful tool in the identification of lymphocyte subpopulations and in the study of the ontogeny and activation of these cells. |
| Databáze: | OpenAIRE |
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