The tobacco gene Ntcyc07 confers arsenite tolerance in Saccharomyces cerevisiae by reducing the steady state levels of intracellular arsenic
| Autor: | Jaekyung Shim, Dong Gwan Kim, Young Geun Mok, Chang Eun Lee, Seong-Ki Kim, Jong Soon Choi, Barry P. Rosen, Joohyun Lee, Young Jin Kim, Hyoung Sun Shin, Byoung-Doo Lee, Yuling Meng, Seongbin Hwang, June Seung Lee |
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| Rok vydání: | 2008 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Arsenites Molecular Sequence Data Saccharomyces cerevisiae Drug Resistance Biophysics S. cerevisiae chemistry.chemical_element Biology Genes Plant Biochemistry Arsenic N. tabaccum chemistry.chemical_compound cyc07 Structural Biology Tobacco Genetics Amino Acid Sequence Cloning Molecular Molecular Biology Gene Arsenite Cloning FPS1 Membrane Proteins Membrane Transport Proteins Cell Biology biology.organism_classification Molecular biology Yeast Repressor Proteins Basic-Leucine Zipper Transcription Factors chemistry ACR1 Steady state (chemistry) ACR3 Intracellular |
| Zdroj: | FEBS Letters. 582:916-924 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2008.02.030 |
| Popis: | We cloned a plant gene, Ntcyc07, conferring arsenite tolerance by expressing a tobacco expression library in WT yeast (Y800). Expression of Ntcyc07 increased the tolerance to As(III) and decreased its accumulation, suggesting that the enhanced As(III) tolerance resulted from a reduction of the intracellular arsenic level. Interestingly, expression of Ntcyc07 increased the expression of the As(III) export carrier ACR3, but repressed that of As(III) uptake channel FPS1. Ntcyc07p interacted with Acr1p, which is the transcriptional activator of ACR3, but not with the ACR3 promoter. Taken together, the data indicated that Ntcyc07p promoted As(III) tolerance by decreasing the intracellular level of As(III) via increasing the expression of ACR3 and reducing that of FPS1. |
| Databáze: | OpenAIRE |
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