Genetic structure and conservation of Mountain Lions in the South-Brazilian Atlantic Rain Forest
Autor: | Camila Schlieper de Castilho, Thales Renato Ochotorena de Freitas, Luiz G. Marins-Sá, Rodrigo C. Benedet |
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Rok vydání: | 2011 |
Předmět: |
Araucaria Forest
microsatellite Linkage disequilibrium Araucaria lcsh:QH426-470 Population Biodiversity Atlantic Rain Forest Effective population size Mata Atlântica Mountain lion Genetic variation Genetics Puma concolor Genetic variability education Molecular Biology Diversidade genética education.field_of_study biology Ecology Conservação conservation genetic diversity biology.organism_classification lcsh:Genetics Genetic structure Research Article |
Zdroj: | Genetics and Molecular Biology, Vol 35, Iss 1, Pp 65-73 (2012) Genetics and Molecular Biology v.35 n.1 2012 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG ResearcherID Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS Genetics and Molecular Biology, Volume: 35, Issue: 1, Pages: 65-73, Published: 15 DEC 2011 |
ISSN: | 1678-4685 1415-4757 |
Popis: | The Brazilian Atlantic Rain Forest, one of the most endangered ecosystems worldwide, is also among the most important hotspots as regards biodiversity. Through intensive logging, the initial area has been reduced to around 12% of its original size. In this study we investigated the genetic variability and structure of the mountain lion, Puma concolor. Using 18 microsatellite loci we analyzed evidence of allele dropout, null alleles and stuttering, calculated the number of allele/locus, PIC, observed and expected heterozygosity, linkage disequilibrium, Hardy-Weinberg equilibrium, F(IS), effective population size and genetic structure (MICROCHECKER, CERVUS, GENEPOP, FSTAT, ARLEQUIN, ONESAMP, LDNe, PCAGEN, GENECLASS software), we also determine whether there was evidence of a bottleneck (HYBRIDLAB, BOTTLENECK software) that might influence the future viability of the population in south Brazil. 106 alleles were identified, with the number of alleles/locus ranging from 2 to 11. Mean observed heterozygosity, mean number of alleles and polymorphism information content were 0.609, 5.89, and 0.6255, respectively. This population presented evidence of a recent bottleneck and loss of genetic variation. Persistent regional poaching constitutes an increasing in the extinction risk. |
Databáze: | OpenAIRE |
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