Microfluidic accumulation assay probes attachment of biofilm forming diatom cells
Autor: | Axel Rosenhahn, Kim A. Nolte, Jana Schwarze |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Biofouling Surface Properties 030106 microbiology Microfluidics Nanotechnology Aquatic Science Biology Applied Microbiology and Biotechnology 03 medical and health sciences Water Movements Marine coatings Water Science and Technology Diatoms Fouling fungi Biofilm Models Theoretical biology.organism_classification Navicula perminuta Diatom Biofilms Biophysics Hydrodynamics Polystyrenes |
DOI: | 10.6084/m9.figshare.5171749.v1 |
Popis: | Testing of fouling release (FR) technologies is of great relevance for discovery of the next generation of protective marine coatings. In this paper, an accumulation assay to test diatom interaction under laminar flow with the model organism Navicula perminuta is introduced. Using time lapse microscopy with large area sampling allows determination of the accumulation kinetics of the diatom on three model surfaces with different surface properties at different wall shear stresses. The hydrodynamic conditions within the flow cell are described and a suitable shear stress range to perform accumulation experiments is identified at which statistically significant discrimination of surfaces is possible. The observed trends compare well to published adhesion preferences of N. perminuta. Also, previously determined trends of critical wall shear stresses required for cell removal from the same set of functionalized interfaces shows consistent trends. Initial attachment mediated by extracellular polymeric substances (EPS) present outside the diatoms leads to the conclusion that the FR potential of the tested coating candidates can be deducted from dynamic accumulation experiments under well-defined hydrodynamic conditions. As well as testing new coating candidates for their FR properties, monitoring of the adhesion process under flow provides additional information on the mechanism and geometry of attachment and the population kinetics. |
Databáze: | OpenAIRE |
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