Synthesis of AZT 5'-triphosphate mimics and their inhibitory effects on HIV-1 reverse transcriptase
| Autor: | Patrick Fagan, Jennifer L. Brooks, Tiffany Hurd, Vasanthakumar Rajappan, Nicholas A. Boyle, P. Dan Cook, Marija Prhavc, Yi Jin, Thomas W. Bruice, Fu Chen, Vivek K. Rajwanshi, Janet M Leeds, Guangyi Wang |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Stereochemistry
Pyrophosphate chemistry.chemical_compound Zidovudine Structure-Activity Relationship Drug Stability Drug Discovery medicine Humans heterocyclic compounds Nucleotide chemistry.chemical_classification biology Reverse-transcriptase inhibitor virus diseases HIV Reverse Transcriptase Enzyme Biochemistry chemistry Enzyme inhibitor Nucleoside triphosphate biology.protein Molecular Medicine Reverse Transcriptase Inhibitors Nucleoside medicine.drug |
| Zdroj: | Journal of medicinal chemistry. 47(27) |
| ISSN: | 0022-2623 |
| Popis: | In search of active nucleoside 5'-triphosphate mimics, we have synthesized a series of AZT triphosphate mimics (AZT P3Ms) and evaluated their inhibitory effects on HIV-1 reverse transcriptase as well as their stability in fetal calf serum and in CEM cell extracts. Reaction of AZT with 2-chloro-4H-1,3,2-benzodioxaphosphorin-4-one, followed by treatment of the phosphite intermediate 2 with pyrophosphate analogues, yielded the cyclic triphosphate intermediates 4b-4f, which were subjected to boronation and subsequent hydrolysis to give AZT 5'-alpha-borano-beta,gamma-bridge-modified triphosphates 6b-6f in moderate to good yields. Reaction of the cyclic intermediate 4d with iodine, followed by treatment with a series of nucleophiles, afforded the AZT 5'-beta,gamma-difluoromethylene-gamma-substituted triphosphates (7b-7i). Several different types of AZT P3Ms containing alpha-P-thio (or dithio) and beta,gamma-difluoromethylene (13,14), alpha,beta-difluoromethylene and gamma-P-methyl(or phenyl) (15,16), and alpha-borano-beta,gamma-difluoromethylene and gamma-O-methyl/phenyl (11,12) were also synthesized. The effectiveness of the compounds as inhibitors of HIV-1 reverse transcriptase was determined using a fluorometric assay and a poly(A) homopolymer as a template. A number of AZT P3Ms exhibited very potent inhibition of HIV-1 reverse transcriptase. Modifications at the beta,gamma-bridge of triphosphate rendered the AZT P3Ms 6b-6f with varied activities (K(i) from 9.5 to >>500 nM) while modification at the alpha,beta-bridge of triphosphate led to weak AZT P3M inhibitors. The results imply that the AZT P3Ms were substrate inhibitors, as is AZT triphosphate. The most active compound, AZT 5'-alpha-R(p)()-borano-beta,gamma-(difluoromethylene)triphosphate (AZT 5'-alphaB-betagammaCF(2)TP) (6d-I), is as potent as AZT triphosphate with a K(i)() value of 9.5 nM and at least 20-fold more stable than AZT triphosphate in the serum and cell extracts. Therefore, for the first time, a highly active and stable nucleoside triphosphate mimic has been identified, which is potentially useful as a new type of antiviral drug. The promising triphosphate mimic, 5'-alpha-borano-beta,gamma-(difluoromethylene)triphosphate, is expected to be valuable to the discovery of nucleotide mimic antiviral drugs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|