Characterization of beta-adrenoceptors in the Syrian hamster Harderian gland: sexual differences and effects of either castration or superior cervical ganglionectomy
| Autor: | Gerald R. Buzzell, Andreas Pangerl, Brigitte Pangerl, David J. Jones, Russel J. Reiter |
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| Rok vydání: | 1989 |
| Předmět: |
Male
Exocrine gland medicine.medical_specialty medicine.medical_treatment Ovariectomy Population Hamster Cellular and Molecular Neuroscience chemistry.chemical_compound Harderian gland Sex Factors Reference Values Internal medicine Cricetinae Receptors Adrenergic beta medicine Animals Ganglionectomy Receptor education education.field_of_study Ganglia Sympathetic biology Mesocricetus Harderian Gland Cell Membrane Lacrimal Apparatus biology.organism_classification Kinetics medicine.anatomical_structure Endocrinology Castration chemistry Pindolol Female Orchiectomy |
| Zdroj: | Journal of neuroscience research. 22(4) |
| ISSN: | 0360-4012 |
| Popis: | Scatchard analysis of saturation isotherms of [125I]-iodopindolol was used to characterize beta-adrenoceptor density (Bmax) and affinity constant (Kd) in female and male hamster Harderian glands. Single-point experiments were also completed in intact females, intact males, and castrated or superior cervical ganglionectomized males. Scatchard analysis described a single population of binding sites with a Bmax of 292.2 +/- 45.1 fmol/mg protein (X +/- SEM, n = 6) in females and 18.2 +/- 3.0 fmol/mg protein (n = 6, P less than .001) in males. The affinity also varied significantly (P less than .05) with a Kd of 1.08 +/- 0.18 versus 0.26 + 0.05 nM (n = 6) in the Harderian gland of females and males, respectively. Single-point [125I]-IPIN (400 pM) binding values in females were 67.3 +/- 4.0, in intact males were 12.8 +/- 3.2, and in castrated males were 31.2 +/- 4.2 fmol/mg protein (n = 7-9). Superior cervical ganglionectomy induced no significant changes in receptor binding. The results indicate pronounced sexual differences in the density and affinity of beta-adrenoceptors in the hamster Harderian gland, which may be sex hormone dependent. |
| Databáze: | OpenAIRE |
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