The transcriptional response of Saccharomyces cerevisiae to Pichia membranifaciens killer toxin
| Autor: | C. Abrusci, Antonio Santos, Maria del Mar Álvarez, Manuel San Mauro, Domingo Marquina |
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| Rok vydání: | 2005 |
| Předmět: |
Transcription
Genetic Saccharomyces cerevisiae Mutant Biochemistry Polymerase Chain Reaction Gene expression Homeostasis Northern blot Molecular Biology Gene Oligonucleotide Array Sequence Analysis biology Gene Expression Profiling Pichia membranifaciens Osmolar Concentration Cell Biology Mycotoxins biology.organism_classification Blotting Northern Phenotype Molecular biology Killer Factors Yeast Real-time polymerase chain reaction Gene Deletion Signal Transduction |
| Zdroj: | The Journal of biological chemistry. 280(51) |
| ISSN: | 0021-9258 |
| Popis: | The transcriptional response of Saccharomyces cerevisiae to Pichia membranifaciens killer toxin (PMKT) was investigated. We explored the global gene expression responses of the yeast S. cerevisiae to PMKT using DNA microarrays, real time quantitative PCR, and Northern blot. We identified 146 genes whose expression was significantly altered in response to PMKT in a non-random functional distribution. The majority of induced genes, most of them related to the high osmolarity glycerol (HOG) pathway, were core environmental stress response genes, showing that the coordinated transcriptional response to PMKT is related to changes in ionic homeostasis. Hog1p was observed to be phosphorylated in response to PMKT implicating the HOG signaling pathway. Individually deleted mutants of both up- (99) and down-regulated genes (47) were studied for altered sensitivity; it was observed that the deletion of up-regulated genes generated hypersensitivity (82%) to PMKT. Deletion of down-regulated genes generated wild-type (36%), resistant (47%), and hypersensitive (17%) phenotypes. This is the first study that shows the existence of a transcriptional response to the poisoning effects of a killer toxin. |
| Databáze: | OpenAIRE |
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