Identification of the High-Virulence Clone of Group B Streptococci by Using a Probe Containing a Putative Aldolase Gene
| Autor: | Brenda C. Timmons, Fortino Solórzano, Elizabeth K. Eskew, S J Mattingly, Gerardo C. Palacios |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Serotype clone (Java method) Genotype Virulence Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Streptococcus agalactiae Fructose-Bisphosphate Aldolase Streptococcal Infections Enzyme Stability medicine Humans Gene Streptococcus Aldolase A Temperature Nucleic Acid Hybridization General Medicine Molecular biology Bacterial Typing Techniques Blotting Southern Restriction enzyme genomic DNA Phenotype nervous system behavior and behavior mechanisms biology.protein DNA Probes hormones hormone substitutes and hormone antagonists |
| Zdroj: | Current Microbiology. 47:0319-0322 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-002-3957-5 |
| Popis: | A high-virulence clone (HVC) was proposed as causing much of the morbidity and mortality when a collection of group B Streptococcus (GBS) isolates was examined by multi-locus enzyme electrophoresis. HVC isolates could be further distinguished by their inability to grow at 40 degrees C, and a temperature-sensitive aldolase was identified as responsible for this characteristic. In the present study, the HVC was sought in a collection of 57 GBS isolates by hybridization with a probe containing a putative aldolase gene on genomic DNA restriction enzyme digests. Isolates were initially classified as HVC or non-HVC by their inability to grow at 40 degrees C. Three serotype III invasive isolates had the HVC control restriction/hybridization pattern. They were also unable to grow at 40 degrees C. The remaining 11 invasive and all carrier isolates showed a pattern identical to that of the non-HVC control. These results provide additional support for the existence of a highly virulent clonal group among serotype III isolates and suggest that hybridization with a probe containing the aldolase gene on DNA restriction enzyme digests can be an alternative method for identifying highly virulent isolates. |
| Databáze: | OpenAIRE |
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