Monoclonal antibodies directed to the blood group A associated structure, galactosyl-A: specificity and relation to the Thomsen-Friedenreich antigen
| Autor: | Henrik Clausen, George Springer, Julia Parker, Mark R. Stroud, Hakomori Sen-Itiroh |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Hemagglutination
medicine.drug_class Immunology Fluorescent Antibody Technique Sialidase Monoclonal antibody Disaccharides ABO Blood-Group System Mice Antigen Antibody Specificity medicine Glycophorin Animals Humans Antigens Tumor-Associated Carbohydrate Molecular Biology Mice Inbred BALB C biology Thomsen-Friedenreich Antigen Mouth Mucosa Antibodies Monoclonal Molecular biology carbohydrates (lipids) biology.protein lipids (amino acids peptides and proteins) Antibody Neuraminidase |
| Zdroj: | Molecular immunology. 25(2) |
| ISSN: | 0161-5890 |
| Popis: | Two monoclonal antibodies, HH8 and HH9, have been established after immunization of mice with galactosyl-A glycolipid antigen having the terminal structure, Gal beta 1----3GalNAc alpha 1----3[Fuc alpha 1----2]Gal beta 1----R, which is the precursor for type 3 chain A (repetitive A) and type 3 chain H (A-associated H). Both antibodies react strongly and specifically with galactosyl-A, but HH8 (IgM) showed strong hemagglutination of blood group A1, A2, O and B erythrocytes after sialidase treatment, while HH9 (IgG1) did not react with human erythrocytes even after sialidase treatment. HH8 and anti-T antibody, but not HH9, reacted with glycophorin A after sialidase treatment. The reactivity of HH8 with glycophorin A was abolished by beta-galactosidase and was inhibited by liposomes containing galactosyl-A, but not other glycolipids. In addition, anti-T antibody and peanut lectin reacted specifically with galactosyl-A glycolipids. These findings indicate that HH8 recognizes the terminal disaccharide Gal beta 1----3GalNAc alpha 1----R, which is the same sequence as the classically known Thomsen-Friedenreich antigen (T-antigen), whereas HH9 does not cross-react with T-antigen but recognizes the entire galactosyl-A structure. The T-antigen was also demonstrated by immunohistology with HH8 after neuraminidase treatment in a subset of cells in stratified epithelium. |
| Databáze: | OpenAIRE |
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