Culture-independent discovery of natural products from soil metagenomes
| Autor: | Bradley M. Hover, Micah Katz, Sean F. Brady |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Secondary Metabolism Bioengineering Computational biology Secondary metabolite Biology 010402 general chemistry 01 natural sciences Applied Microbiology and Biotechnology Soil 03 medical and health sciences chemistry.chemical_compound Drug Discovery medicine Humans Secondary metabolism Soil Microbiology Biological Products Natural product Bacteria business.industry Drug discovery DNA Biosynthetic Pathways 0104 chemical sciences Biotechnology 030104 developmental biology chemistry Metagenomics Metagenome Heterologous expression business Soil microbiology Culture independent medicine.drug |
| Zdroj: | Journal of Industrial Microbiology and Biotechnology. 43:129-141 |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1007/s10295-015-1706-6 |
| Popis: | Bacterial natural products have proven to be invaluable starting points in the development of many currently used therapeutic agents. Unfortunately, traditional culture-based methods for natural product discovery have been deemphasized by pharmaceutical companies due in large part to high rediscovery rates. Culture-independent, or “metagenomic,” methods, which rely on the heterologous expression of DNA extracted directly from environmental samples (eDNA), have the potential to provide access to metabolites encoded by a large fraction of the earth’s microbial biosynthetic diversity. As soil is both ubiquitous and rich in bacterial diversity, it is an appealing starting point for culture-independent natural product discovery efforts. This review provides an overview of the history of soil metagenome-driven natural product discovery studies and elaborates on the recent development of new tools for sequence-based, high-throughput profiling of environmental samples used in discovering novel natural product biosynthetic gene clusters. We conclude with several examples of these new tools being employed to facilitate the recovery of novel secondary metabolite encoding gene clusters from soil metagenomes and the subsequent heterologous expression of these clusters to produce bioactive small molecules. |
| Databáze: | OpenAIRE |
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