Taenia saginata: polymerase chain reaction for taeniasis diagnosis in human fecal samples
| Autor: | Cáris Maroni Nunes, Rodrigo Norberto Pereira, José Fernando Garcia, Mauro Massaharu Nakano, Luiz Gustavo Ferraz Lima, Camila Santos Manoel |
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| Přispěvatelé: | Universidade Estadual Paulista (UNESP) |
| Rok vydání: | 2003 |
| Předmět: |
Immunology
Biology Polymerase Chain Reaction law.invention Feces Species Specificity law parasitic diseases Taenia solium medicine Taeniasis Animals Humans Polymerase chain reaction DNA Primers Electrophoresis Agar Gel Carrier state Zoonosis Taenia saginata General Medicine DNA Helminth medicine.disease biology.organism_classification Virology DNA extraction medicine.drug_formulation_ingredient Infectious Diseases Carrier State Taenia Parasitology |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0014-4894 |
| Popis: | Made available in DSpace on 2022-04-28T19:56:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-01-01 The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T. saginata identification in human fecal samples. © 2003 Elsevier Science (USA). All rights reserved. Department of Animal Health and Production Universidade Estadual Paulista Júlio de Mesquita Filho Campus Aracatuba, Rua Clóvis Pestana, 793, Araçatuba, SP 16050-680 Department of Animal Health and Production Universidade Estadual Paulista Júlio de Mesquita Filho Campus Aracatuba, Rua Clóvis Pestana, 793, Araçatuba, SP 16050-680 |
| Databáze: | OpenAIRE |
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