Lineage-specific rapid diagnostic tests can resolve Trypanosoma cruzi TcII/V/VI ecological and epidemiological associations in the Argentine Chaco
Autor: | Michael A. Miles, Pascal Mertens, Ricardo E. Gürtler, Quentin Gilleman, Niamh Murphy, M. Victoria Cardinal, Natalia Paula Macchiaverna, Yves Gustin, Tapan Bhattacharyya, Nicolas Zeippen |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Chagas disease Armadillos Trypanosoma cruzi 030231 tropical medicine Population Prevalence Argentina Antibodies Protozoan Enzyme-Linked Immunosorbent Assay Lineage-specific Rapid diagnostic test Serogroup Serology lcsh:Infectious and parasitic diseases 03 medical and health sciences Dogs 0302 clinical medicine Seroepidemiologic Studies parasitic diseases medicine Animals Humans Seroprevalence Serologic Tests lcsh:RC109-216 education education.field_of_study biology Diagnostic Tests Routine Research medicine.disease biology.organism_classification Virology 3. Good health Cross-Sectional Studies 030104 developmental biology Infectious Diseases Parasitology Cats ELISA Trypanosomiasis |
Zdroj: | Parasites & Vectors, Vol 12, Iss 1, Pp 1-11 (2019) Parasites & Vectors |
ISSN: | 1756-3305 |
Popis: | Background Trypanosoma cruzi, the protozoan agent of Chagas disease, is comprised of at least 6 genetic lineages (TcI-TcVI). Their geographical distribution, clinical associations and reservoir hosts are not fully elucidated, as genotyping is hampered due to the difficulty in isolating representative populations of organisms. Lineage-specific serological techniques may address these issues. Methods Trypanosoma cruzi lineage-specific serological assays were performed on human, canine, feline and armadillo sera from the Gran Chaco in northern Argentina, a region of ongoing transmission. Synthetic peptides representing lineage-specific epitopes of the trypomastigote small surface antigen (TSSA) were used in ELISA, and the TcII/V/VI shared epitope peptide (TSSApep-II/V/VI) was used in the Chagas Sero K-SeT rapid diagnostic test (RDT). Results Chagas Sero K-SeT RDT, using Protein G to detect human and canine IgG, was at least as sensitive as TSSApep-II/V/VI ELISA using specific secondary antibodies. For sera from humans TSSApep-II/V/VI seroprevalence by Chagas Sero K-SeT was 273/393 (69.5%), for dogs 48/73 (65.8%) and for armadillos 1/7 (14.3%); by ELISA for cats 5/19 (26.3%). The seroprevalence for humans was similar to that for Bolivian patients, amongst whom we previously observed an association of TSSApep-II/V/VI seropositivity with severity of cardiomyopathy. In humans, prevalence of TSSApep-II/V/VI recognition was associated with locality, and with increasing and decreasing age within the Qom and Creole populations, respectively. For dogs TSSApep-II/V/VI recognition was associated with being born before community-wide insecticide spraying (P = 0.05) and with Qom household (P Conclusions We show here that Chagas Sero K-SeT RDT can replace ELISA for TSSApep-II/V/VI serology of humans and dogs; for humans there were statistically significant associations between a positive Chagas Sero K-SeT RDT and being resident in Area IV, and for dogs association with Qom household or with being born before the mass spraying campaign; we also show that with cats the TcII/V/VI epitope can be detected by ELISA. We assessed the lineage distribution in an unprecedented 83% of the human T. cruzi-seropositive population. These results form the basis for more detailed studies, enabling rapid in-the-field surveillance of the distribution and clustering of these lineages among humans and mammalian reservoirs of T. cruzi infection. |
Databáze: | OpenAIRE |
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