A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method
| Autor: | Sara Capistrós, Jesús Yániz, S. Vicente-Fiel, Carlos O Hidalgo, Pilar Santolaria |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male endocrine system BOAR Sperm Head Swine Urology Nuclear area Semen Invited Original Article lcsh:RC870-923 fluorescence microscopy sperm morphometry Andrology 03 medical and health sciences 0302 clinical medicine Agglutinin image analysis Image Processing Computer-Assisted Animals Acrosome Ovis Cell Shape reproductive and urinary physiology artiodactyls 030219 obstetrics & reproductive medicine Sheep biology urogenital system General Medicine Anatomy biology.organism_classification lcsh:Diseases of the genitourinary system. Urology Sperm Spermatozoa computer-assisted sperm morphometry analysis 030104 developmental biology Microscopy Fluorescence Cattle |
| Zdroj: | Asian Journal of Andrology, Vol 18, Iss 6, Pp 840-843 (2016) Asian Journal of Andrology Zaguán. Repositorio Digital de la Universidad de Zaragoza instname |
| ISSN: | 1745-7262 |
| Popis: | The aim of this study was to compare the sperm nuclear and acrosomal morphometry of three species of domestic artiodactyls; cattle (Bos taurus), sheep (Ovis aries), and pigs (Sus scrofa). Semen smears of twenty ejaculates from each species were fixed and labeled with a propidium iodide-Pisum sativum agglutinin (PI/PSA) combination. Digital images of the sperm nucleus, acrosome, and whole sperm head were captured and analyzed. The use of the PI/PSA combination and CASA-Morph fluorescence-based method allowed the capture, morphometric analysis, and differentiation of most sperm nuclei, acrosomes and whole heads, and the assessment of acrosomal integrity with a high precision in the three species studied. For the size of the head and nuclear area, the relationship between the three species may be summarized as bull > ram > boar. However, for the other morphometric parameters (length, width, and perimeter), there were differences in the relationships between species for sperm nuclei and whole sperm heads. Bull sperm acrosomes were clearly smaller than those in the other species studied and covered a smaller proportion of the sperm head. The acrosomal morphology, small in the bull, large and broad in the sheep, and large, long, and with a pronounced equatorial segment curve in the boar, was species-characteristic. It was concluded that there are clear variations in the size and shape of the sperm head components between the three species studied, the acrosome being the structure showing the most variability, allowing a clear distinction of the spermatozoa of each species. |
| Databáze: | OpenAIRE |
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