Insertional Mutation of the Drosophila Nuclear Lamin Dm0 Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae
| Autor: | Bertram Schmitt, Rita Reifegerste, Heinrich Betz, Erich Buchner, Silke Fuchs, Bettina Lenz-Böhme, Jasmine Wismar |
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| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Cytoplasm
Embryo Nonmammalian Annulate lamella Nuclear Envelope Mutant Molecular Sequence Data Genes Insect Biology Article Transformation Genetic medicine Animals Drosophila Proteins Nuclear pore Fluorescent Antibody Technique Indirect Mitosis Germ-Line Mutation Brain Chemistry Base Sequence Homozygote Gene Expression Regulation Developmental Nuclear Proteins Cell Biology Microtomy Molecular biology Introns Lamins Cell nucleus Microscopy Electron Mutagenesis Insertional medicine.anatomical_structure Fertility Phenotype Nuclear lamina Drosophila Lamin Locomotion |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Nuclear lamins are thought to play an important role in disassembly and reassembly of the nucleus during mitosis. Here, we describe a Drosophila lamin Dm 0 mutant resulting from a P element insertion into the first intron of the Dm 0 gene. Homozygous mutant animals showed a severe phenotype including retardation in development, reduced viability, sterility, and impaired locomotion. Immunocytochemical and ultrastructural analysis revealed that reduced lamin Dm 0 expression caused an enrichment of nuclear pore complexes in cytoplasmic annulate lamellae and in nuclear envelope clusters. In several cells, particularly the densely packed somata of the central nervous system, defective nuclear envelopes were observed in addition. All aspects of the mutant phenotype were rescued upon P element-mediated germline transformation with a lamin Dm 0 transgene. These data constitute the first genetic proof that lamins are essential for the structural organization of the cell nucleus. |
| Databáze: | OpenAIRE |
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