Pigment Melanin Scavenges Nitric Oxide In Vitro: Possible Relevance to Keloid Formation
| Autor: | Julian M. Menter, Mohamed A. Bayorh, Ahmad M. Dawaghreh, Danita Eatman, Isaac Willis |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
In situ
integumentary system lcsh:QD450-801 lcsh:Physical and theoretical chemistry Photochemistry In vitro Nitric oxide Adduct Dialysis tubing lcsh:Chemistry Melanin chemistry.chemical_compound Pigment lcsh:QD1-999 chemistry visual_art visual_art.visual_art_medium Organic chemistry lcsh:Q Physical and Theoretical Chemistry Sepia lcsh:Science |
| Zdroj: | Research Letters in Physical Chemistry, Vol 2008 (2008) |
| ISSN: | 1687-6881 1687-6873 |
| Popis: | Recently, nitric oxide (NO) has been implicated in the formation of keloids, preferentially formed in dark-skinned persons, and we suspected that pigment melanin itself may play a direct role by adsorbing NO. We tested the ability of cuttlefish sepia melanin to scavenge (adsorb) NO, generated in situ by 2-(N.N Diethylamino) diazeneolate-2-oxide (DEA/NO), through a dialysis membrane. NO was measured as NO2_ and NO3_ by the Griess method and as N2O3 by trapping experiments with the fluorogenic substrate 4,5-diaminofluorescein (DAF-2). Initial NO2_ and NO3_ concentrations were significantly lower in the test dialyzates than in controls. Scavenging of NO was rapid enough to compete with DAF adduct formation. Both analytical methods gave comparable results. Adsorbed NO and/or its oxidized products may undergo interactions with melanin, adsorbed O2, and/or dermal material that may lead to keloid formation. |
| Databáze: | OpenAIRE |
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