Molecular characterization and xenogenic application of Wharton's jelly derived caprine mesenchymal stem cells
| Autor: | D. N. Madhu, G. Saikumar, Amarpal, M. D. Pratheesh, Pawan K. Dubey, Bhaskar Sharma, Nitin E. Gade, Amar Nath, T. B. Sivanarayanan, G. Taru Sharma |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Cell Biology Stem cell marker Andrology Random Allocation Wharton's jelly medicine Animals Von Kossa stain Cells Cultured Cell Proliferation Wound Healing General Veterinary Goats Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine In vitro Staining medicine.anatomical_structure Immunology Antigens Surface Heterografts Rabbits Wound healing |
| Zdroj: | Veterinary research communications. 38(2) |
| ISSN: | 1573-7446 |
| Popis: | Aim of the present study was in vitro expansion and characterization of caprine wharton’s jelly derived mesenchymal stem cells (cWJ-MSCs) to investigate their tissue healing potential in xenogenic animal model. Plastic adherent fibroblastoid cell populations with distinctive homogeneous morphology were isolated from caprine wharton’s jelly explants. These wharton’s jelly derived cells were found positive for the surface markers CD-73, STRO-1 and CD-105, whereas they were negative for hematopoetic stem cell marker CD-34. In vitro cultured cWJ-MSCs also showed differentiation properties into osteogenic, adipogenic and chondrogenic lineages as demonstrated by von Kossa, Oil Red- O and Alcian blue staining respectively, which was further confirmed and quantified by flow cytometric analysis. Furthermore, these well characterized cWJ-MSCs were evaluated for the wound-healing potential in full-thickness skin wounds in rabbit model for 28 days. Caprine WJ- MSCs treated skin wounds showed significantly (P |
| Databáze: | OpenAIRE |
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