Presence of HPV DNA in convalescent salivary rinses is an adverse prognostic marker in head and neck squamous cell carcinoma
| Autor: | Steven S. Chang, Patrick K. Ha, Wayne M. Koch, Joseph A. Califano, Alice Y. Chuang, Shaoyu Zhou, Tony C. Y. Chuang, William H. Westra, Shahnaz Begum |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Cancer Research Saliva Pathology medicine.medical_specialty Sensitivity and Specificity Gastroenterology Article law.invention law Internal medicine Biomarkers Tumor medicine Humans Oral mucosa Polymerase chain reaction Aged Neoplasm Staging Human papillomavirus 16 business.industry Papillomavirus Infections Head and neck cancer virus diseases Cancer Middle Aged Prognosis medicine.disease Head and neck squamous-cell carcinoma female genital diseases and pregnancy complications medicine.anatomical_structure Real-time polymerase chain reaction Oncology Head and Neck Neoplasms DNA Viral Carcinoma Squamous Cell Feasibility Studies Papilloma Female Neoplasm Recurrence Local Oral Surgery business |
| Zdroj: | Oral Oncology. 44:915-919 |
| ISSN: | 1368-8375 |
| DOI: | 10.1016/j.oraloncology.2008.01.001 |
| Popis: | Summary Human papillomavirus (HPV) 16 is present in up to 60% of patients with head and neck squamous cell carcinoma (HNSCC) and confers a favorable prognosis in terms of recurrence and mortality. Previous reports demonstrated that HPV-16 DNA can be detected in the initial salivary rinses from these patients. In this study, we assessed the feasibility of post-treatment HPV DNA shed from the oral mucosa as a prognostic marker for persistent/recurrent head and neck cancer. Fresh tumor samples and pre- and post-treatment salivary rinses were collected from 59 patients with HNSCC. HPV-16 E6 and E7 DNA copy number in these samples were quantified by real time PCR. Twenty of 59 patients (33.9%) were HPV-16 positive in their tumors before treatment. Four of 20 HPV tumor positive patients ultimately developed recurrence, and two of these four patients were HPV-16 positive in surveillance salivary rinses (sensitivity = 50%). Of the 39 (66.1%) HPV-16 negative patients on initial clinical presentation and the 16 HPV-16 positive patients who did not recur, none were HPV-16 positive in salivary rinses after treatment (specificity = 100%). HPV-16 presence in follow-up salivary rinses preceded clinical detection of disease recurrence by an average of 3.5 months. Patients with presence of HPV-16 DNA in surveillance salivary rinses are at significant risk for recurrence. Quantitative measurement of salivary HPV-16 DNA has promise for surveillance and early detection of recurrence. |
| Databáze: | OpenAIRE |
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