Calcium transport in human salivary glands: a proposed model of calcium secretion into saliva
| Autor: | Veronika Homann, Evamaria Kinne-Saffran, Rolf K. H. Kinne, Peter Gaengler, Wolfgang H. Arnold |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Histology Submandibular Gland chemistry.chemical_element TRPV Cation Channels Calcium Models Biological Calcium in biology Sarcoplasmic Reticulum Calcium-Transporting ATPases Plasma Membrane Calcium-Transporting ATPases S100 Calcium Binding Protein G stomatognathic system Calcium-binding protein Humans Parotid Gland Saliva Molecular Biology Calcium signaling Calcium metabolism Reverse Transcriptase Polymerase Chain Reaction Calcium channel Calcium-Binding Proteins Cell Biology Immunohistochemistry Cell biology Calcium ATPase Medical Laboratory Technology Biochemistry chemistry Gene Expression Regulation Calbindin 2 Plasma membrane Ca2+ ATPase Calcium Channels |
| Zdroj: | Histochemistry and cell biology. 125(5) |
| ISSN: | 0948-6143 |
| Popis: | Salivary calcium plays a vital role in bio-mineralization of dental enamel and exposed dentin. In order to elucidate the yet unknown cellular and molecular mechanisms of calcium secretion in human salivary glands the presence of various relevant plasma membrane transport systems for calcium were investigated. Using an RT-PCR approach, expression of the epithelial calcium channel (CaT-Like), the calcium binding protein (calbindin-2), the endoplasmic reticulum pumps (SERCA-2 and -3), and the plasma membrane calcium ATPases (PMCA-1, -2, and -4), were found in parotid and submandibular glands. Immunohistochemistry revealed that CaT-Like is located in the basolateral plasma membrane of acinar cells; while calbindin-2, SERCA-2 and SERCA-3 were found inside the acinar cells; and PMCA-2 was found in the apical membrane and in the secretory canaliculi between the cells. Based on these findings, we propose the following model of calcium secretion in human salivary glands: (1) calcium enters the acinar cell at the basolateral side via calcium channel CaT-Like (calcium influx); (2) intracellular calcium is taken up into the endoplasmic reticulum by SERCA-2 and possibly SERCA3 or bound to calbindin-2 (intracellular calcium pool); and (3) calcium is secreted by PMCAs at the apical plasma membrane (calcium efflux). |
| Databáze: | OpenAIRE |
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