Circulating Polymorphonuclear Leukocytes from Patients with Gram-Negative Bacteremia Are Not Primed for Enhanced Production of Leukotriene B4 or 5-Hydroxyeicosatetraenoic Acid
| Autor: | George L. May, Tania C. Sorrell, Krystyna Sztelma |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Gram-negative bacteria Neutrophils Leukotriene B4 Neutrophile Bacteremia Pathogenesis chemistry.chemical_compound Hydroxyeicosatetraenoic Acids medicine Humans Immunology and Allergy Calcimycin Sensitization 5-Hydroxyeicosatetraenoic acid Aged Aged 80 and over biology Hydroxyeicosatetraenoic acid hemic and immune systems Middle Aged medicine.disease biology.organism_classification Endotoxins Infectious Diseases medicine.anatomical_structure chemistry Immunology Female lipids (amino acids peptides and proteins) Gram-Negative Bacterial Infections circulatory and respiratory physiology |
| Zdroj: | The Journal of Infectious Diseases. 169:1151-1154 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/169.5.1151 |
| Popis: | The hypothesis was tested that polymorphonuclear leukocytes (PMNL) from patients with gram-negative bacteremia are primed to produce leukotriene B4 (LTB4) or 5-hydroxyeicosatetraenoic acid (5-HETE), in response to concentrations of calcium ionophore A23187, which are substimulatory for control PMNL. PMNL from 11 bacteremic patients and 8 healthy subjects (11 samples) produced similar quantities of LTB4, omega-oxidation products of LTB4, and 5-HETE after incubation with 0.3 and 0.5 microM A23187 for 5 min. At the detection threshold of 0.3 microM A23187, LTB4 was present in PMNL preparations from 9 of 11 patients and 7 of 11 control samples and 5-HETE from the same 9 patients and from 6 controls. There was no correlation between LTB4 or 5-HETE and plasma levels of endotoxin. In this group of patients, priming of PMNL by gram-negative bacteremia did not lead to enhanced production of LTB4, its omega-oxidation products, or 5-HETE when PMNL were challenged with low concentrations of A23187. |
| Databáze: | OpenAIRE |
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