Use of Ribotyping To Retrospectively Identify Methicillin-Resistant Staphylococcus aureus Isolates from Phase 3 Clinical Trials for Tigecycline That Are Genotypically Related to Community-Associated Isolates
| Autor: | Paul M. Dunman, Patricia A. Bradford, Guy Singh, Fionnuala McAleese, Timothy Babinchak, John O'Connell, Battouli Saïd-Salim, Ellen Murphy, Steven J. Projan, Barry N. Kreiswirth |
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| Rok vydání: | 2005 |
| Předmět: |
Staphylococcus aureus
Micrococcaceae Minocycline Microbial Sensitivity Tests Tigecycline medicine.disease_cause Ribotyping Microbiology Mechanisms of Resistance medicine Cluster Analysis Humans Pharmacology (medical) Retrospective Studies Antibacterial agent Pharmacology biology business.industry SCCmec Clindamycin biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Methicillin-resistant Staphylococcus aureus Community-Acquired Infections Infectious Diseases Methicillin Resistance business medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 49:4521-4529 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.49.11.4521-4529.2005 |
| Popis: | A retrospective study was performed to identify methicillin-resistant Staphylococcus aureus (MRSA) isolates obtained from patients enrolled in phase 3 clinical trials for tigecycline that were genotypically similar to known community-associated MRSA (CA-MRSA) strains. The clinical trials were double-blind comparator studies for complicated skin and skin structure infections or complicated intra-abdominal infections. We obtained 85% of the MRSA isolates from patients with complicated skin and skin structure infections. Using ribotyping, MRSA isolates were compared with well-characterized North American CA-MRSA strains and negative-control hospital-associated (HA) MRSA strains by cluster analysis; 91 of the 173 isolates clustered with two groups of known CA-MRSA strains, 60% of which shared an indistinguishable ribotype. These isolates were subsequently tested for the presence of SCC mec type IV and the Panton-Valentine leukocidin (PVL)-encoding genes as well as susceptibility to clindamycin, characteristics that are typically associated with CA-MRSA; 89 of the 91 isolates carried the type IV SCC mec element and 76 were also positive for the PVL-encoding genes; 73 of these isolates were susceptible to clindamycin. A similar analysis performed on 26 nonclustering isolates identified only four with these characteristics; 89 of the 91 clustering isolates were inhibited by tigecycline at MICs of ≤0.5 μg/ml. On the basis of clustering information and preliminary genetic characterization, it appears that ribotyping is a useful tool in identifying potential CA-MRSA isolates and 76 MRSA isolates from patients enrolled in the tigecycline phase 3 trials have genetic markers typically associated with CA-MRSA. |
| Databáze: | OpenAIRE |
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