Revealing the developmental dynamics in male strobilus transcriptome of Gnetum luofuense using nanopore sequencing technology
| Autor: | Shengqing Shi, Boxiang He, Chen Hou, Yuxin Tian, Dongcheng Liang, Huiming Lian, Wang Yingli, Nan Deng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pollination Gnetum Science Real-Time Polymerase Chain Reaction 01 natural sciences Article Strobilus Flowering Transcriptome 03 medical and health sciences Gymnosperm Pollinator Gene Expression Regulation Plant Plant development Gene Multidisciplinary biology Plant morphogenesis Gene Expression Regulation Developmental biology.organism_classification Nanopore Sequencing 030104 developmental biology Evolutionary biology Pollen Medicine RNA Long Noncoding Adaptation 010606 plant biology & botany Transcription Factors |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Gnetum is a pantropical distributed gymnosperm genus. As being dioecious, Gnetum species apply female and male strobili to attract and provide nutrition to insect pollinators. Due to its unique gross morphology, a Gnetum male strobilus receives much attention in previous taxonomic and evolutionary studies. However, underlying molecular mechanisms that control male strobilus development and pollination adaptation have not been well studied. In the present study, nine full-length transcriptomes were sequenced from three developmental stages of the G. luofuense male strobili using Oxford Nanopore Technologies. In addition, weighted gene co-expression network analysis (WGCNA), and RT-qPCR analysis were performed. Our results show that a total of 3138 transcription factors and 466 long non-coding RNAs (lncRNAs) were identified, and differentially expressed lncRNAs and TFs reveal a dynamic pattern during the male strobilus development. Our results show that MADS-box and Aux/IAA TFs were differentially expressed at the three developmental stages, suggesting their important roles in the regulation of male strobilus development of G. luofuense. Results of WGCNA analysis and annotation of differentially expressed transcripts corroborate that the male strobilus development of G. luofuense is closely linked to plant hormone changes, photosynthesis, pollination drop secretion and reproductive organ defense. Our results provide a valuable resource for understanding the molecular mechanisms that drive organ evolution and pollination biology in Gnetum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |