Sequence Recombination and Conservation of Varroa destructor Virus-1 and Deformed Wing Virus in Field Collected Honey Bees (Apis mellifera)
| Autor: | Robert D. Possee, Jinmin Ma, D. Pallett, Hui Wang, Linda A. King, Tim G. Shreeve, Jiazheng Xie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Varroidae
Population lcsh:Medicine Insect Viruses Genome Viral DNA sequencing Deep sequencing Conserved sequence 03 medical and health sciences symbols.namesake Deformed wing virus Animals Wings Animal RNA Small Interfering education lcsh:Science Conserved Sequence 030304 developmental biology Genetics Sanger sequencing Recombination Genetic 0303 health sciences education.field_of_study Multidisciplinary biology Base Sequence 030306 microbiology Chimera lcsh:R Reproducibility of Results Sequence Analysis DNA Bees biology.organism_classification Biology and Microbiology GenBank Varroa destructor symbols lcsh:Q Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 9, p e74508 (2013) |
| ISSN: | 1932-6203 |
| Popis: | We sequenced small (s) RNAs from field collected honeybees (Apis mellifera) and bumblebees ( Bombus pascuorum ) using the Illumina technology. The sRNA reads were assembled and resulting contigs were used to search for virus homologues in GenBank. Matches with Varroa destructor virus-1 (VDV1) and Deformed wing virus (DWV) genomic sequences were obtained for A. mellifera but not B . pascuorum . Further analyses suggested that the prevalent virus population was composed of VDV-1 and a chimera of 5’-DWV-VDV1-DWV-3’. The recombination junctions in the chimera genomes were confirmed by using RT-PCR, cDNA cloning and Sanger sequencing. We then focused on conserved short fragments (CSF, size > 25 nt) in the virus genomes by using GenBank sequences and the deep sequencing data obtained in this study. The majority of CSF sites confirmed conservation at both between-species (GenBank sequences) and within-population (dataset of this study) levels. However, conserved nucleotide positions in the GenBank sequences might be variable at the within-population level. High mutation rates (Pi>10%) were observed at a number of sites using the deep sequencing data, suggesting that sequence conservation might not always be maintained at the population level. Virus-host interactions and strategies for developing RNAi treatments against VDV1/DWV infections are discussed. |
| Databáze: | OpenAIRE |
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