Lineage tracing of Sox2-expressing progenitor cells in the mouse inner ear reveals a broad contribution to non-sensory tissues and insights into the origin of the organ of Corti
| Autor: | Rende Gu, Tegy J. Vadakkan, Andrew K. Groves, Tiantian Cai, Mary E. Dickinson, Alyssa L. Crowder, Victor G. Piazza, Rogers M. Brown, Chih-Wei Hsu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell type Population Mice Transgenic Biology Article Mice 03 medical and health sciences Imaging Three-Dimensional Neural Stem Cells stomatognathic system SOX2 Genes Reporter Fate mapping otorhinolaryngologic diseases medicine Animals Cell Lineage Inner ear Gene Knock-In Techniques education Organ of Corti Molecular Biology Cochlea education.field_of_study Receptors Notch SOXB1 Transcription Factors Gene Expression Regulation Developmental Cell Biology Anatomy Antigens Differentiation Embryonic stem cell Cell biology Luminescent Proteins 030104 developmental biology medicine.anatomical_structure Ear Inner embryonic structures sense organs biological phenomena cell phenomena and immunity Jagged-1 Protein Signal Transduction Developmental Biology |
| Zdroj: | Developmental Biology. 414:72-84 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2016.03.027 |
| Popis: | The transcription factor Sox2 is both necessary and sufficient for the generation of sensory regions of the inner ear. It regulates expression of the Notch ligand Jag1 in prosensory progenitors, which signal to neighboring cells to up-regulate Sox2 and sustain prosensory identity. However, the expression pattern of Sox2 in the early inner ear is very broad, suggesting that Sox2-expressing progenitors form a wide variety of cell types in addition to generating the sensory regions of the ear. We used Sox2-CreER mice to follow the fates of Sox2-expressing cells at different stages in ear development. We find that Sox2-expressing cells in the early otocyst give rise to large numbers of non-sensory structures throughout the inner ear, and that Sox2 only becomes a truly prosensory marker at embryonic day (E)11.5. Our fate map reveals the organ of Corti derives from a central domain on the medial side of the otocyst and shows that a significant amount of the organ of Corti derives from a Sox2-negative population in this region. |
| Databáze: | OpenAIRE |
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