Optimization of ectoine production from Nesterenkonia xinjiangensis and one-step ectoine purification
| Autor: | Furkan Orhan, Ertuğrul Ceyran, Akın Akincioğlu |
|---|---|
| Přispěvatelé: | Belirlenecek |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
Cation exchange
ion exchange incubation time Nesterenkonia xinjiangensis Nitrogen compounds nitrogen response surface methodology 'current Nitrogen sources Bacterial cells glucose Waste Management and Disposal Sulfur compounds fermentation Purification pH RSM xylose sucrose ion exchange chromatography General Medicine bacterium Chlorine compounds diamino acid Positive ions sodium chloride ammonium sulfate Optimisations optimization cell extract Environmental Engineering experimental design culture medium Nesterenkonia bacterium culture Bioengineering concentration (parameter) Cell extracts chemistry response surface method Article lactose ectoine process optimization ammonium acetate Cation exchanges nonhuman Renewable Energy Sustainability and the Environment carbon mannitol Amino Acids Diamino Nesterenkonium Response-surface methodology ammonium chloride bacterial cell Culture Media carbon source ammonium nitrate dry weight maltose metabolism |
| Popis: | In the current study, the optimization of ectoine production by Nesterenkonia xinjiangensis and purification of ectoine from the bacterial cell extract were performed for the first time. Various carbon sources (glucose, sucrose, maltose, lactose, mannitol, and xylose) and nitrogen sources (ammonium nitrate, ammonium phosphate, ammonium chloride, ammonium oxalate, ammonium sulphate, and ammonium acetate), were used to optimize ectoine production. Subsequently, the effects of salt, pH and, concentrations of carbon and nitrogen source on ectoine production were optimized by response surface methodology (RSM). Ultimately, high pure (over 99%) and yield (98%) of ectoine from bacterial cells extracted was obtained by a single-step process using cation exchange chromatography. This study provides information that higher ectoine production can be achieved from this bacterial isolate by optimizing the factors influencing ectoine production and thus can be used as a new and alternative ectoine producer. © 2023 BAP- FEF.20.001 This study was supported by Agri Ibrahim Cecen University, Scientific Researches Project (Project number: BAP- FEF.20.001). |
| Databáze: | OpenAIRE |
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