hCLE/C14orf166, a cellular protein required for viral replication, is incorporated into influenza virus particles
| Autor: | Amelia Nieto, Juan Ortín, Maite Pérez-Cidoncha, Alejandra Pazo, Alicia Pérez-González, Alejandro Roldan-Gomendio, Sara Landeras-Bueno, Susana de Lucas, Laura Marcos-Villar, Ariel Rodriguez-Frandsen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cytoplasm Immunoelectron microscopy viruses RNA polymerase II Virus Replication medicine.disease_cause Article Virus Madin Darby Canine Kidney Cells Viral Proteins 03 medical and health sciences Dogs Influenza A Virus H1N1 Subtype Influenza A Virus H9N2 Subtype Influenza A virus medicine Animals Humans Microscopy Immunoelectron Polymerase Ribonucleoprotein Cell Nucleus Multidisciplinary biology Influenza A Virus H3N2 Subtype Virion RNA Virology 3. Good health HEK293 Cells 030104 developmental biology Gene Expression Regulation Ribonucleoproteins Viral replication A549 Cells Host-Pathogen Interactions Proteolysis Trans-Activators biology.protein |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Scientific Reports |
| Popis: | The influenza A virus polymerase associates with a number of cellular transcription-related factors, including the RNA polymerase II (RNAP II). We previously described that the cellular protein hCLE/ C14orf166 interacts with and stimulates influenza virus polymerase as well as RNAP II activities. Here we show that, despite the considerable cellular shut-off observed in infected cells, which includes RNAP II degradation, hCLE protein levels increase throughout infection in a virus replication-dependent manner. Human and avian influenza viruses of various subtypes increase hCLE levels, but other RNA or DNA viruses do not. hCLE colocalises and interacts with viral ribonucleoproteins (vRNP) in the nucleus, as well as in the cytoplasm late in infection. Furthermore, biochemical analysis of purified virus particles and immunoelectron microscopy of infected cells show hCLE in virions, in close association with viral vRNP. These findings indicate that hCLE, a cellular protein important for viral replication, is one of the very few examples of transcription factors that are incorporated into particles of an RNA-containing virus. This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO; BFU2011-26175 and BFU2014-57797-R) and the Ciber de Enfermedades Respiratorias (ISCIII). |
| Databáze: | OpenAIRE |
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