Atrial Natriuretic Peptide—The Influence of Various Physiological and Sampling Conditions
| Autor: | H. B. Benraad, Th J Benraad, B G A Theelen, F. M. A. Rosmalen, A. C. I. T. L. Tan, Peter W. C. Kloppenborg |
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| Rok vydání: | 1987 |
| Předmět: |
Adult
Male 030213 general clinical medicine medicine.medical_specialty Posture Clinical Biochemistry Radioimmunoassay 030209 endocrinology & metabolism Peptide hormone 03 medical and health sciences 0302 clinical medicine Atrial natriuretic peptide Pregnancy Internal medicine Humans Medicine Sampling (medicine) Menstrual Cycle Blood Specimen Collection business.industry General Medicine Circadian Rhythm Endocrinology Female business Atrial Natriuretic Factor |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 24:500-507 |
| ISSN: | 1758-1001 0004-5632 |
| Popis: | A study of various physiological conditions, possibly influencing levels of atrial natriuretic peptide (ANP), is described. Atrial natriuretic peptide was determined by a radioimmunoassay, suitable for routine measurements, using 1 mL of human plasma. Atrial naturetic peptide was adsorbed onto Sep-pak C-18 cartridges, eluted with acidified ethanol and subsequently radioimmunoassayed. The detection limit was slightly less than 2 pg/tube (approximately 5 pg/mL plasma). Intra- and interassay coefficients of variation were 7 and 10%, respectively. Study of various sampling conditions revealed that blood sampling in EDTA-tubes kept on ice, and centrifuged within 1 h, gives the most reliable results. Reference values in 74 individuals ranged between 10 to 69 pg/mL (mean +/- SD = 30 +/- 11 pg/mL). We observed no difference in reference values when the blood sampling procedure was in the sitting (29 +/- 11 pg/mL, n = 43) or supine (31 +/- 12 pg/mL, n = 31) position. Venepuncture stress did not consistently change ANP-levels. No difference was observed in ANP between the follicular and the luteal phase of the menstrual cycle. ANP levels of 7 ambulant female subjects declined significantly during the study period from 7 a.m. to 3 p.m. |
| Databáze: | OpenAIRE |
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