Prohibitin involvement in the generation of mitochondrial superoxide at complex I in human sperm

Autor: Guo-Wu Chen, Wai-Sum O, Patricia A. Martin-DeLeon, Hong Chen, Hui‐Juan Shi, Ran-Ran Chai
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Mitochondrial ROS
Adult
Male
medicine.medical_specialty
endocrine system
prohibitin
Matrix metalloproteinase
Biology
mitochondrial ROS
Lipid peroxidation
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Superoxides
Internal medicine
Prohibitins
medicine
Humans
Prohibitin
Sperm motility
reproductive and urinary physiology
Membrane potential
Membrane Potential
Mitochondrial
030219 obstetrics & reproductive medicine
Electron Transport Complex I
Sperm Count
urogenital system
technology
industry
and agriculture
Cell Biology
Original Articles
Sperm
Spermatozoa
Mitochondria
Repressor Proteins
human sperm
030104 developmental biology
Endocrinology
chemistry
Mitochondrial Membrane Protein
Sperm Motility
Molecular Medicine
lipids (amino acids
peptides
and proteins)
Original Article
Lipid Peroxidation
Reactive Oxygen Species
mitochondrial complex I
Zdroj: Journal of Cellular and Molecular Medicine
ISSN: 1582-4934
1582-1838
Popis: Prohibitin (PHB), a major mitochondrial membrane protein, has been shown earlier in our laboratoryto regulate sperm motility via an alteration in mitochondrial membrane potential (MMP) in infertile men with poor sperm quality. To test if PHB expression is associated with sperm mitochondrial superoxide (mROS) levels, here we examined sperm mROS levels, high MMP and lipid peroxidation in infertile men with poor sperm motility (asthenospermia, A) and/or low sperm concentrations (oligoasthenospermia, OA). The diaphorase‐type activity of sperm mitochondrial complex I (MCI) and PHB expression were also determined. We demonstrate that mROS and lipid peroxidation levels are significantly higher in sperm from A and OA subjects than in normospermic subjects, whereas high MMP and PHB expression are significantly lower. A positive correlation between mROS and lipid peroxidation and a negative correlation of mROS with PHB expression, high MMP, and sperm motility were found in these subjects. The finding of similar diaphorase‐type activity levels of sperm MCI in the three groups studied suggests that the catalytic subunits of MCI in the matrix arm may produce mROS on its own. There may be a dysfunction of electron transport at MCI associated with decreased expression of PHB in sperm with poor quality. We conclude that mROS level is increased and associated with decreased PHB expression, and it may regulate sperm motility via increases in low MMP and lipid peroxidation. This is the first report on the involvement of PHB in human sperm motility loss associated with increased generation of mROS at MCI.
Databáze: OpenAIRE
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