Antithromboplastin in Human Serum and Plasma
| Autor: | C. Symons, T. G. Scott, R. L. Markham |
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| Rok vydání: | 1960 |
| Předmět: | |
| Zdroj: | Nature. 186:248-249 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/186248a0 |
| Popis: | THE existence of antithromboplastin as a blood coagulation factor has been well documented (for example, see ref. 1). While it is likely that antithromboplastin plays a part in the prevention of blood coagulation in the body in health, it does not appear to have been characterized physiologically. Berry2 has indicated a suitable technique for its demonstration which we have applied with slight modifications in detail only to whole serum, whole plasma (platelet-free) and also to the electrophoretic fractions of serum and plasma. These fractions were eluted by mechanical squeezing from strips cut from pieces 36-cm. wide of Whatman No. 3 paper used in a ‘fakir's mattress’ type of electrophoresis apparatus3. Our technique was, basically, to incubate serum, plasma or the electrophoretic fractions with human brain thromboplastin and calcium chloride at 37° C., and at known intervals to add an aliquot of this incubation mixture to a substrate platelet-free plasma and note the time it takes to clot. A saline control repeatedly gave coagulation times of about 20 sec. (shown as 100 units on the ordinate in Fig. 1). It should be noted that this technique demonstrates antithromboplastin active only against the extrinsic tissue type of thromboplastin and not against the thromboplastin formed by blood undergoing the natural coagulation mechanisms (intrinsic thromboplastin). |
| Databáze: | OpenAIRE |
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