Detection of Serum miRNAs Affecting Liver Apoptosis in a Periodontitis Rat Model
| Autor: | Toshiki Yoneda, Yoshio Sugiura, Hisataka Miyai, Kohei Fujimori, Manabu Morita, Daisuke Ekuni, Takayuki Maruyama, Takaaki Tomofuji, Terumasa Kobayashi |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty medicine.medical_treatment Rat model Apoptosis General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Internal medicine microRNA medicine Animals RNA Messenger Rats Wistar Periodontitis Ligature Pharmacology Messenger RNA business.industry Microarray analysis techniques medicine.disease Rats Disease Models Animal MicroRNAs Endocrinology Liver 030220 oncology & carcinogenesis Serum mirna business Research Article |
| Zdroj: | In Vivo. 34:117-123 |
| ISSN: | 1791-7549 0258-851X |
| Popis: | Background/Aim: miRNA molecules have been attracting attention as genetic modifiers between organs. We examined the relationship between serum miRNA and targeted liver mRNA profiles in a periodontitis rat model, and the influence of periodontitis on the liver. Materials and Methods: Male Wistar rats (n=16, 8 weeks old) were randomly divided into two groups (8 rats each): control and periodontitis (ligature placement for 4 weeks). Serum miRNA and liver mRNA profiles were compared. Results: Periodontal destruction and hepatocyte apoptosis were induced in the periodontitis group. Microarray analysis indicated that 52 serum miRNAs and 33 liver mRNAs were expressed with a >1.5-fold change (FC) and a >2.0-FC (p2.0-FC, among which, Hyou1, Chac1, and Bloc1s3 have apoptotic functions in our model. miRNAs upstream of these 3 mRNAs are miR-3591, miR-181a-2-3p and miR-6321. Conclusion: miR-3591, miR-181a-2-3p and miR-6321 induced hepatocyte apoptosis in our periodontitis rat model. |
| Databáze: | OpenAIRE |
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