Vasoactive intestinal peptide regulates ileal goblet cell production in mice

Autor: Luke A. Schwerdtfeger, Stuart A. Tobet
Rok vydání: 2019
Předmět:
Male
Physiology
Vasoactive intestinal peptide
Peripherins
Enteroendocrine cell
Tetrodotoxin
030204 cardiovascular system & hematology
digestive system
lcsh:Physiology
Signalling Pathways
03 medical and health sciences
Mice
0302 clinical medicine
Intestinal mucosa
Physiology (medical)
medicine
Neural Circuits and Systems
Animals
intestine
mucosa
Cell Proliferation
organotypic
Neurons
Goblet cell
lcsh:QP1-981
Cell growth
Chemistry
goblet cell
Vasoactive intestinal peptide receptor
Peripherin
Original Articles
respiratory system
Mucus
3. Good health
Cell biology
Mice
Inbred C57BL
VIP
medicine.anatomical_structure
Gastrointestinal
Hepatic and Pancreatic Physiology
Receptors
Vasoactive Intestinal Peptide
Female
Original Article
Goblet Cells
030217 neurology & neurosurgery
Vasoactive Intestinal Peptide
Zdroj: Physiological Reports
Physiological Reports, Vol 8, Iss 3, Pp n/a-n/a (2020)
ISSN: 2051-817X
Popis: Innervation of the intestinal mucosa has gained more attention with demonstrations of tuft and enteroendocrine cell innervation. However, the role(s) these fibers play in maintaining the epithelial and mucus barriers are still poorly understood. This study therefore examines the proximity of mouse ileal goblet cells to neuronal fibers, and the regulation of goblet cell production by vasoactive intestinal peptide (VIP). An organotypic intestinal slice model that maintains the cellular diversity of the intestinal wall ex vivo was used. An ex vivo copper‐free click‐reaction to label glycosaminoglycans was used to identify goblet cells. Pharmacological treatment of slices was used to assess the influence of VIP receptor antagonism on goblet cell production and neuronal fiber proximity. Goblet cells were counted and shown to have at least one peripherin immunoreactive fiber within 3 µm of the cell, 51% of the time. Treatment with a VIP receptor type I and II antagonist (VPACa) resulted in an increase in the percentage of goblet cells with peripherin fibers. Pharmacological treatments altered goblet cell counts in intestinal crypts and villi, with tetrodotoxin and VPACa substantially decreasing goblet cell counts. When cultured with 5‐Ethynyl‐2’‐deoxyuridine (EdU) as an indicator of cell proliferation, colocalization of labeled goblet cells and EdU in ileal crypts was decreased by 77% when treated with VPACa. This study demonstrates a close relationship of intestinal goblet cells to neuronal fibers. By using organotypic slices from mouse ileum, vasoactive intestinal peptide receptor regulation of gut wall goblet cell production was revealed.
A close relationship between neuronal fibers and enteric goblet cells was demonstrated, with more neuronal fibers being in close proximity to goblet cells in vasoactive intestinal peptide (VIP) receptor antagonized ileum slices. Further, antagonizing VIP receptors ex vivo was shown to regulate goblet cell production in the ileal crypt.
Databáze: OpenAIRE
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